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N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies
Li D.2; Liu J.4; Zhang L.4; Xu T.4; Chen J.4; Wang L.3; Zhao Q.4
2015-12-01
Source PublicationVirologica Sinica
ISSN1995820X 16740769
Volume30Issue:6Pages:449-456
Abstract

The Human immunodeficiency virus type 1 (HIV-1) gp41 membrane proximal external region (MPER) is targeted by broadly neutralizing antibodies (e.g. 2F5, 4E10, Z13e and m66.6), which makes this region a promising target for vaccine design. One strategy to elicit neutralizing antibodies against the MPER epitope is to design peptide immunogens mimicking neutralization structures. To probe 2F5-like neutralizing antibodies, two yeast-displayed antibody libraries from peripheral blood mononuclear cells from a HIV-1 patient were screened against the 2F5 epitope peptide SP62. Two 2F5-like antibodies were identified that specifically recognized SP62. However, these antibodies only weakly neutralized HIV-1 primary isolates. The epitopes recognized by these two 2F5-like antibodies include not only the 2F5 epitope (amino acids (aa) 662–667 in the MPER) but also several other residues (aa 652–655) locating at the N-terminus in SP62. Experimental results suggest that residues of SP62 adjacent to the 2F5 epitope influence the response of broadly neutralizing 2F5-like antibodies in vaccination. Our findings may aid the design of vaccine immunogens and development of therapeutics against HIV-1 infection.[Figure not available: see fulltext.]

Keyword2f5 Hiv-1 Membrane Proximal External Region (Mper) Neutralizing Antibody Yeast Display
DOI10.1007/s12250-015-3664-6
URLView the original
Indexed BySCI
Language英语
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000367520100007
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Cited Times [WOS]:5   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Universidade de Macau
2.Xiamen University
3.Jilin University
4.Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li D.,Liu J.,Zhang L.,et al. N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies[J]. Virologica Sinica,2015,30(6):449-456.
APA Li D..,Liu J..,Zhang L..,Xu T..,Chen J..,...&Zhao Q..(2015).N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies.Virologica Sinica,30(6),449-456.
MLA Li D.,et al."N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies".Virologica Sinica 30.6(2015):449-456.
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