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Studies on aminoglycoside susceptibility identify a novel function of KsgA to secure translational fidelity during antibiotic stress
Zou J.2; Zhang W.2; Zhang H.2; Zhang X.D.2; Peng B.3; Zheng J.2
2018-10-01
Source PublicationAntimicrobial Agents and Chemotherapy
ISSN10986596 00664804
Volume62Issue:10
Abstract

Antibiotic resistance has become a global crisis. Studies on the mechanism of bacterial tolerance to antibiotics will not only increase our conceptual understanding of bacterial death but also provide potential targets for novel inhibitors. We screened a mutant library containing a full set of in-frame deletion mutants of Escherichia coli K-12 and identified 140 genes that possibly contribute to gentamicin tolerance. The deletion of ksgA increased the inhibition and killing potency against mid-log-phase bacteria by aminoglycosides. Initially identified as a 16S rRNA methyltransferase, KsgA also has additional functions as a ribosomal biogenesis factor and a DNA glycosylase. We found that the methyltransferase activity of KsgA is responsible for the tolerance, as demonstrated by a site-directed mutagenesis analysis. In contrast to the mechanism for cold sensitivity, the decreased tolerance to aminoglycoside is not related to the failure of ribosomal biogenesis. Furthermore, the DNA glycosylase activity of KsgA contributes minimally to kanamycin tolerance. Importantly, we discovered that KsgA secures protein translational fidelity upon kanamycin killing, in contrast to its role during cold stress and kasugamycin treatment. The results suggest that the compromise in protein translational fidelity in the absence of KsgA is the root cause of an increased sensitivity to a bactericidal aminoglycoside. In addition, KsgA in the pathogenic Acinetobacter baumannii contributes not only to the tolerance against aminoglycoside killing but also to virulence in the host, warranting its potential application as a target for inhibitors that potentiate aminoglycoside therapeutic killing as well as disarm bacterial virulence simultaneously.

KeywordAcinetobacter Baumannii Aminoglycosides Ksga Translational Fidelity Virulence
DOI10.1128/AAC.00853-18
URLView the original
Indexed BySCI
Language英语
WOS Research AreaMicrobiology ; Pharmacology & Pharmacy
WOS SubjectMicrobiology ; Pharmacology & Pharmacy
WOS IDWOS:000445405500031
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Cited Times [WOS]:1   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Qingdao National Laboratory for Marine Science and Technology
2.Universidade de Macau
3.Sun Yat-Sen University
Recommended Citation
GB/T 7714
Zou J.,Zhang W.,Zhang H.,et al. Studies on aminoglycoside susceptibility identify a novel function of KsgA to secure translational fidelity during antibiotic stress[J]. Antimicrobial Agents and Chemotherapy,2018,62(10).
APA Zou J.,Zhang W.,Zhang H.,Zhang X.D.,Peng B.,&Zheng J..(2018).Studies on aminoglycoside susceptibility identify a novel function of KsgA to secure translational fidelity during antibiotic stress.Antimicrobial Agents and Chemotherapy,62(10).
MLA Zou J.,et al."Studies on aminoglycoside susceptibility identify a novel function of KsgA to secure translational fidelity during antibiotic stress".Antimicrobial Agents and Chemotherapy 62.10(2018).
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