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Structure-guided disruption of the pseudopilus tip complex inhibits the Type II secretion in Pseudomonas aeruginosa
Zhang Y.2; Faucher F.2; Zhang W.1; Wang S.3; Neville N.2; Poole K.2; Zheng J.1; Jia Z.2
2018-10-01
Source PublicationPLoS Pathogens
ISSN15537374 15537366
Volume14Issue:10
Abstract

Pseudomonas aeruginosa utilizes the Type II secretion system (T2SS) to translocate a wide range of large, structured protein virulence factors through the periplasm to the extracellular environment for infection. In the T2SS, five pseudopilins assemble into the pseudopilus that acts as a piston to extrude exoproteins out of cells. Through structure determination of the pseudopilin complexes of XcpVWX and XcpVW and function analysis, we have confirmed that two minor pseudopilins, XcpV and XcpW, constitute a core complex indispensable to the pseudopilus tip. The absence of either XcpV or -W resulted in the non-functional T2SS. Our small-angle X-ray scattering experiment for the first time revealed the architecture of the entire pseudopilus tip and established the working model. Based on the interaction interface of complexes, we have developed inhibitory peptides. The structure-based peptides not only disrupted of the XcpVW core complex and the entire pseudopilus tip in vitro but also inhibited the T2SS in vivo. More importantly, these peptides effectively reduced the virulence of P. aeruginosa towards Caenorhabditis elegans.

DOI10.1371/journal.ppat.1007343
URLView the original
Indexed BySCI
WOS Research AreaMicrobiology ; Parasitology ; Virology
WOS SubjectMicrobiology ; Parasitology ; Virology
WOS IDWOS:000448978700027
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Universidade de Macau
2.Queen's University, Kingston
3.Beijing Normal University
Recommended Citation
GB/T 7714
Zhang Y.,Faucher F.,Zhang W.,et al. Structure-guided disruption of the pseudopilus tip complex inhibits the Type II secretion in Pseudomonas aeruginosa[J]. PLoS Pathogens,2018,14(10).
APA Zhang Y..,Faucher F..,Zhang W..,Wang S..,Neville N..,...&Jia Z..(2018).Structure-guided disruption of the pseudopilus tip complex inhibits the Type II secretion in Pseudomonas aeruginosa.PLoS Pathogens,14(10).
MLA Zhang Y.,et al."Structure-guided disruption of the pseudopilus tip complex inhibits the Type II secretion in Pseudomonas aeruginosa".PLoS Pathogens 14.10(2018).
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