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Calycosin rebalances advanced glycation end products-induced glucose uptake dysfunction of hepatocyte in vitro
Xu Y.1; Xiong J.1; Zhao Y.1; He B.2; Zheng Z.2; Chu G.1; Zhu Q.1
2015
Source PublicationAmerican Journal of Chinese Medicine
ISSN0192415X
Volume43Issue:6Pages:1191-1210
Abstract

Diabetes mellitus (DM) often accompanies liver dysfunction. Astragali Radix is a traditional Chinese herbal medicine that is widely administrated to ameliorate the symptoms of diabetes as well as liver dysfunction, but its acting mechanism is still not yet fully recognized. Advanced glycation end products (AGEs) play a key role in promoting diabetic organ dysfunction. Both hyperglycemia and AGEs can induce insulin resistance, hepatocyte damage and liver dysfunction. We designed this study to explore the effects of the phytoestrogen Calycosin, a major active component of Astragali Radix, on AGEs-induced glucose uptake dysfunction in the hepatocyte cell line and relevant mechanisms. MTT and BrdU methods were applied to evaluate cell viability. 2-NBDG was used to observe glucose uptake by a live cell imaging system. Immunofluorescence method was carried out to investigate GLUT1, GLUT4, and RAGE protein expressions on cell membrane. cAMP content was determined by an EIA method. We found Calycosin concentration-dependently ameliorated AGEs-induced hepatocyte viability damage. AGEs dramatically reduced basal glucose uptake in hepatocytes, and this reduction could be reversed by Calycosin administration. By immunofluorescence detection, we observed that Calycosin could inhibit AGEs-induced GLUT1 expression down-regulation via estrogen receptor (ER). Furthermore, Calycosin decreased AGEs-promoted RAGE and cAMP elevation in hepatocytes. These findings strongly suggest that Calycosin can ameliorate AGEs-promoted glucose uptake dysfunction in hepatocytes; the protection of cell viability and ER-RAGE and GLUT1 pathways play a significant role in this modulation.

KeywordAdvanced Glycation End Products Calycosin Glucose Uptake Hepatocyte Radix Astragali
DOI10.1142/S0192415X15500688
URLView the original
Indexed BySCI
Language英语
WOS Research AreaIntegrative & Complementary Medicine ; General & Internal Medicine
WOS SubjectIntegrative & Complementary Medicine ; Medicine, General & Internal
WOS IDWOS:000362666600008
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Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorXu Y.; Zhu Q.
Affiliation1.Macau University of Science and Technology
2.Institute of Consun Co. for Chinese Medicine in Kidney Diseases
Recommended Citation
GB/T 7714
Xu Y.,Xiong J.,Zhao Y.,et al. Calycosin rebalances advanced glycation end products-induced glucose uptake dysfunction of hepatocyte in vitro[J]. American Journal of Chinese Medicine,2015,43(6):1191-1210.
APA Xu Y..,Xiong J..,Zhao Y..,He B..,Zheng Z..,...&Zhu Q..(2015).Calycosin rebalances advanced glycation end products-induced glucose uptake dysfunction of hepatocyte in vitro.American Journal of Chinese Medicine,43(6),1191-1210.
MLA Xu Y.,et al."Calycosin rebalances advanced glycation end products-induced glucose uptake dysfunction of hepatocyte in vitro".American Journal of Chinese Medicine 43.6(2015):1191-1210.
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