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Kuwanon G preserves LPS-induced disruption of gut epithelial barrier in vitro
Guo H.1; Xu Y.1; Huang W.1; Zhou H.1; Zheng Z.2; Zhao Y.1; He B.2; Zhu T.1; Tang S.1; Zhu Q.1
Source PublicationMolecules
AbstractDefects in the gut epithelial barrier have now been recognized to be responsible for diabetic endotoxemia. In everyday life, Mulberry leaf tea is widely used in Asian nations due to its proposed benefits to health and control of diabetes. Evidence indicates the potential role of Kuwanon G (KWG), a component from Morus alba L., on blocking the gut epithelial barrier. In lipopolysaccharides (LPS)-damaged Caco-2 cells, it was found that KWG increased the viability of cells in a concentration-dependent manner. KWG administration significantly elevated the anti-oxidant abilities via increasing ratio of superoxidase dismutase (SOD)/malondialdehyde (MDA) and decreasing reactive oxygen species (ROS) within the cells. During KWG incubation, pro-inflammatory cytokines including interleukin (IL)-1β and tumor necrosis factor (TNF)-α were significantly reduced, tight junction proteins including zonula occludens (ZO)-1, intercellular adhesion molecule (ICAM)-1 and Occludin were dramatically increased as detected by immunofluorescence assay, trans-epithelial electrical resistance was significantly increased and the transmission of albumin-fluorescein isothiocyanate (FITC) across the barrier was decreased. In conclusion, the present study demonstrated that KWG could ameliorate LPS-induced disruption of the gut epithelial barrier by increasing cell viability and tight junction between cells, and decreasing pro-inflammatory cytokines and oxidative damage.
KeywordDiabetes mellitus Gut epithelial barrier Inflammation Kuwanon G Morus alba L.
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Cited Times [WOS]:11   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Macau University of Science and Technology
2.Institute of Consun Co. for Chinese Medicine in Kidney Diseases
3.Macau Institute for Applied Research in Medicine and Health
4.Southwest Medical University
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GB/T 7714
Guo H.,Xu Y.,Huang W.,et al. Kuwanon G preserves LPS-induced disruption of gut epithelial barrier in vitro[J]. Molecules,2016,21(11).
APA Guo H..,Xu Y..,Huang W..,Zhou H..,Zheng Z..,...&Zhu Q..(2016).Kuwanon G preserves LPS-induced disruption of gut epithelial barrier in vitro.Molecules,21(11).
MLA Guo H.,et al."Kuwanon G preserves LPS-induced disruption of gut epithelial barrier in vitro".Molecules 21.11(2016).
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