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Antagonism of Ca2+ influx via L-type Ca2+ channels mediates the vasorelaxant effect of catharanthus roseus-derived vindorosine in rat renal artery
Wu X.-L.1; Cheang W.S.2; Zhang D.-M.1; Li Y.1; Lau C.-W.2; Wang G.-C.1; Huang Y.2; Ye W.-C.1
2014
Source PublicationPlanta Medica
ISSN14390221 00320943
Volume80Issue:18Pages:1672-1677
Abstract

Catharanthus roseus is a traditional herbal medicine used in Asian and African countries for the treatment of various diseases including hypertension. The present study examined possible cellular mechanisms for the relaxation of rat renal arteries induced by vindorosine extracted from C. roseus. Intrarenal arteries were isolated from 200-300 g male Sprague-Dawley rats and treated with different pharmacological blockers and inhibitors for the measurement of vascular reactivity on a Multi Myograph System. Fluorescence imaging by laser scanning confocal microscopy was utilized to determine the intracellular Ca level in the vascular smooth muscles of the renal arteries. Vindorosine in micromolar concentrations relaxes renal arteries precontracted by KCl, phenylephrine, 11-dideoxy-9α,11α-epoxymethanoprostaglandin F2α, and serotonin. Vindorosine-induced relaxations were unaffected by endothelium denudation or by treatment with the nitric oxide synthase inhibitor N -nitro-L-arginine methyl ester hydrochloride, the guanylyl cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, the cyclooxygenase inhibitor indomethacin, or K channel blockers such as tetraethylammonium ions, glibenclamide, and BaCl. Vindorosine-induced relaxations were attenuated in the presence of 0.1 μM nifedipine (an L-type Ca channel blocker). Vindorosine also concentration-dependently suppressed contractions induced by CaCl (0.01-5 mM) in Ca-free 60 mM KCl solution. Furthermore, fluorescence imaging using fluo-4 demonstrated that 30 min incubation with 100 μM vindorosine reduced the 60 mM KCl-stimulated Ca influx in the smooth muscles of rat renal arteries. The present study is probably the first report of blood vessel relaxation by vindorosine and the possible underlying mechanisms involving the inhibition of Ca entry via L-type Ca channels in vascular smooth muscles.

KeywordApocynaceae Ca2++++ Channel Catharanthus Roseus Renal Artery Vasorelaxation Vindorosine
DOI10.1055/s-0034-1383220
URLView the original
Indexed BySCIE
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS IDWOS:000346411300002
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Cited Times [WOS]:2   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Jinan University
2.Chinese University of Hong Kong
Recommended Citation
GB/T 7714
Wu X.-L.,Cheang W.S.,Zhang D.-M.,et al. Antagonism of Ca2+ influx via L-type Ca2+ channels mediates the vasorelaxant effect of catharanthus roseus-derived vindorosine in rat renal artery[J]. Planta Medica,2014,80(18):1672-1677.
APA Wu X.-L..,Cheang W.S..,Zhang D.-M..,Li Y..,Lau C.-W..,...&Ye W.-C..(2014).Antagonism of Ca2+ influx via L-type Ca2+ channels mediates the vasorelaxant effect of catharanthus roseus-derived vindorosine in rat renal artery.Planta Medica,80(18),1672-1677.
MLA Wu X.-L.,et al."Antagonism of Ca2+ influx via L-type Ca2+ channels mediates the vasorelaxant effect of catharanthus roseus-derived vindorosine in rat renal artery".Planta Medica 80.18(2014):1672-1677.
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