UM
Rapid and visual detection of aflatoxin B1 in foodstuffs using aptamer/G-quadruplex DNAzyme probe with low background noise
Wang L.1; Zhu F.1; Chen M.1; Zhu Y.1; Xiao J.2; Yang H.1; Chen X.1
2019-01-15
Source PublicationFood Chemistry
ISSN18737072 03088146
Volume271Pages:581-587
AbstractContamination of foods by aflatoxin B1 (AFB1) is a common serious problem. To improve the efficiency of AFB1 detection, this study aims to develop a sensitive aptasensor for detection of AFB1 in food samples based on the catalytic effect of aptamer/G-quadruplex DNAzyme probe. The resulting reassembly of this probe in the presence of hemin and K catalyzes the generation of fluorescent 2,3-diaminophenazine (DAP) from o-phenylenediamine (OPD). Interestingly, we first found that the high background induced by the superfluous hemin can be effectively suppressed with the aid of sequential adsorption and magnetic separation by magnetic oxidized multiwall carbon nanotubes (FeO@oMWCNTs). This aptasensor exhibits a high sensitivity toward AFB1 with a detection limit of 0.02 ng/mL. The assay also shows higher selectivity for AFB1 compared to other reported agents and can be employed to detect AFB1 in foodstuffs, which might find broad practical applications in other food contaminants determination.
KeywordAflatoxin B1 Aptamer DNAzyme Fluorescence Foodstuffs Magnetic oxidized multiwall carbon nanotubes
DOI10.1016/j.foodchem.2018.08.007
URLView the original
Language英語
Fulltext Access
Citation statistics
Cited Times [WOS]:5   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Central South University China
2.Universidade de Macau
Recommended Citation
GB/T 7714
Wang L.,Zhu F.,Chen M.,et al. Rapid and visual detection of aflatoxin B1 in foodstuffs using aptamer/G-quadruplex DNAzyme probe with low background noise[J]. Food Chemistry,2019,271:581-587.
APA Wang L..,Zhu F..,Chen M..,Zhu Y..,Xiao J..,...&Chen X..(2019).Rapid and visual detection of aflatoxin B1 in foodstuffs using aptamer/G-quadruplex DNAzyme probe with low background noise.Food Chemistry,271,581-587.
MLA Wang L.,et al."Rapid and visual detection of aflatoxin B1 in foodstuffs using aptamer/G-quadruplex DNAzyme probe with low background noise".Food Chemistry 271(2019):581-587.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang L.]'s Articles
[Zhu F.]'s Articles
[Chen M.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang L.]'s Articles
[Zhu F.]'s Articles
[Chen M.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang L.]'s Articles
[Zhu F.]'s Articles
[Chen M.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.