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Induction of ferroptosis and mitochondrial dysfunction by oxidative stress in PC12 cells
Wu, Chuanhong1; Zhao, Wenwen1; Yu, Jie1; Li, Shaojing2; Lin, Ligen1; Chen, Xiuping1
2018-01-12
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume8
Abstract

Neurodegenerative diseases (NDD) are typically associated with neuron loss in nervous system areas. Interventions with related death mechanisms may ameliorate NDD progression. Oxidative stress plays an important role in NDD cell death routines. However, tert-butylhydroperoxide (t-BHP), a widely used oxidative stress stimulus, induces neural cell death through a mechanism that remains elusive. In our study, the ferroptosis marker events occurred after co-treatment with 100 mu M t-BHP for 1 h, all of which were reversed in the presence of the ferroptosis inhibitor ferrostatin-1 (Fer-1) and the iron chelator deferoxamine, implying the occurrence of ferroptosis. Moreover, mitochondria ! dysfunction accompanied by a decreased in membrane potential and ATP production, increased mitochondrial ROS generation. Furthermore, this mitochondrial dysfunction could be reversed by Fer-1. In addition, JNK1/2 and ERK1/2 were activated upstream of the ferroptosis and mitochondrial dysfunction. In summary, these data suggest that ferroptosis, coupled with mitochondrial dysfunction, was involved in t-BHP-induced PC12 death. JNK1/2 and ERK1/2 played important roles in t-BHP-induced cell death. Overall, this study might provide clues to the oxidative stress-based strategies for cell protection in NDD.

DOIhttp://doi.org/10.1038/s41598-017-18935-1
URLView the original
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000419945400015
PublisherNATURE PUBLISHING GROUP
The Source to ArticleWOS
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Cited Times [WOS]:13   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorChen, Xiuping
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
2.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Wu, Chuanhong,Zhao, Wenwen,Yu, Jie,et al. Induction of ferroptosis and mitochondrial dysfunction by oxidative stress in PC12 cells[J]. SCIENTIFIC REPORTS,2018,8.
APA Wu, Chuanhong,Zhao, Wenwen,Yu, Jie,Li, Shaojing,Lin, Ligen,&Chen, Xiuping.(2018).Induction of ferroptosis and mitochondrial dysfunction by oxidative stress in PC12 cells.SCIENTIFIC REPORTS,8.
MLA Wu, Chuanhong,et al."Induction of ferroptosis and mitochondrial dysfunction by oxidative stress in PC12 cells".SCIENTIFIC REPORTS 8(2018).
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