UM
Extraction and determination of major hypotensive compounds in bark of Eucommia ulmoides Oliv.
Xiao J.; Wei X.; Wang Y.
2009-12-01
Source PublicationArchives of Biological Sciences
ISSN03544664
Volume61Issue:4Pages:811-817
AbstractA reversed-phase liquid chromatographic method was developed for the quantitative determination of three major hypotensive compounds, namely geniposidic acid, chlorogenic acid, and geniposide in the bark of Eucommia ulmoides. Soxhlet extraction of GPA, GPS, and CA from E. ulmoides was optimized according to the Taguchi experimental design. Maximum global yields were obtained using the following conditions: extraction temperature, 80°C; extraction time, 1 h; number of extractions, three; solvent volume, 16 ml/g of sample; and 50% ethanol concentration in water. Optimal conditions of separation and detection were achieved on a Diamonsil ODS C18 column (150 mm × 4.6 mm, 5 μm) with a linear gradient of methanol and 0.04% aqueous phosphoric acid (v/v) at a flow rate of 1.0 ml/min and detection wavelength of 240 nm. All calibration curves showed good linearity (r2 > 0.999) within test ranges. The relative deviation of this method was less than 3% for intra- and inter-day assays, and the recovery percentage of the method was 95-104%, with a relative standard deviation (R.S.D.) of less than 5%. The current assay method was used for quantitative determination of geniposidic acid, chlorogenic acid, and geniposide in five samples of E. ulmoides with different age. The results indicate that the developed method could be readily utilized as a quality control method in working with E. ulmoides.
KeywordChina Chlorogenic acid Eucommia ulmoides Geniposide Geniposidic acid HPLC
DOI10.2298/ABS0904811X
URLView the original
Language英語
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Cited Times [WOS]:5   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
AffiliationShanghai Normal University
Recommended Citation
GB/T 7714
Xiao J.,Wei X.,Wang Y.. Extraction and determination of major hypotensive compounds in bark of Eucommia ulmoides Oliv.[J]. Archives of Biological Sciences,2009,61(4):811-817.
APA Xiao J.,Wei X.,&Wang Y..(2009).Extraction and determination of major hypotensive compounds in bark of Eucommia ulmoides Oliv..Archives of Biological Sciences,61(4),811-817.
MLA Xiao J.,et al."Extraction and determination of major hypotensive compounds in bark of Eucommia ulmoides Oliv.".Archives of Biological Sciences 61.4(2009):811-817.
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