UM  > 中華醫藥研究院
Determination of tea polysaccharides in Camellia sinensis by a modified phenol-sulfuric acid method
Xi X.; Wei X.; Wang Y.; Chu Q.; Xiao J.
2010-08-06
Source PublicationArchives of Biological Sciences
ISSN03544664
Volume62Issue:3Pages:669-676
Abstract

A direct procedure for the determination of total polysaccharides (TPS) in Camellia sinensis was set up based on the modified phenol-sulfuric acid method. The monosaccharide composition of TPS was analyzed by GC. Based on the results of GC, model monosaccharide mixtures were made which provided an adequate standard for this procedure. Through single-factor and orthogonal (L3) experiments, the experimental conditions such as the volume of phenol, the volume of concentrated sulfuric acid, the reaction time, and the incubation temperature, were optimized. The highest sensitivity of absorbance was obtained when the volume of concentrated sulfuric acid, the volume of phenol (6%), and the incubation temperature were 2.5 ml, 0.2 ml, and 50C, respectively. Under optimum conditions, the prepared samples were determined satisfactorily, with the recovery from 100.2% to 103.7%, and a relative standard deviation (RSD) of 2.1%. Overall, the modified method is easily operated, rapid, sensitive and accurate. A similar procedure can be applied to the determination of other plant polysaccharides as well.

KeywordDetermination Phenol-sulfuric Tea Polysaccharides
DOI10.2298/ABS1003669X
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaLife Sciences & Biomedicine - Other Topics
WOS SubjectBiology
WOS IDWOS:000281482000021
Fulltext Access
Citation statistics
Cited Times [WOS]:35   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
AffiliationShanghai Normal University
Recommended Citation
GB/T 7714
Xi X.,Wei X.,Wang Y.,et al. Determination of tea polysaccharides in Camellia sinensis by a modified phenol-sulfuric acid method[J]. Archives of Biological Sciences,2010,62(3):669-676.
APA Xi X.,Wei X.,Wang Y.,Chu Q.,&Xiao J..(2010).Determination of tea polysaccharides in Camellia sinensis by a modified phenol-sulfuric acid method.Archives of Biological Sciences,62(3),669-676.
MLA Xi X.,et al."Determination of tea polysaccharides in Camellia sinensis by a modified phenol-sulfuric acid method".Archives of Biological Sciences 62.3(2010):669-676.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xi X.]'s Articles
[Wei X.]'s Articles
[Wang Y.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xi X.]'s Articles
[Wei X.]'s Articles
[Wang Y.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xi X.]'s Articles
[Wei X.]'s Articles
[Wang Y.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.