UM
Molecular cloning and characterization of glutamate decarboxylase CDNA from the giant-embryo Oryza sativa
Xu H.; Liao P.; Xiao J.; Zhang Q.; Dong Y.; Kai G.
2010-12-01
Source PublicationArchives of Biological Sciences
ISSN03544664
Volume62Issue:4Pages:873-879
AbstractA full-length cDNA encoding glutamate decarboxylase (designated as OsGAD3), which catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA), was isolated from the GABA-rich giant-embryo Oryza sativa (Shangshi Jing 315). The full-length cDNA of OsGAD3 (SSJ315) has a 1479 bp open reading frame (ORF) encoding a protein of 492 amino acid residues. The deduced protein had an isoelectric point (pI) of 5.72 and a calculated molecular weight of 56.1 kD. Sequence comparison showed that OsGAD3 (SSJ315) matches the glutamate decarboxylases of other plant species reported previously. Analysis of the structural features of the C-terminal portions of plant GADs revealed that OsGAD3 (SSJ315) has the typical CaM-binding domain (CaMBD) in the C-terminal region as most other plant GADs. Evolution analysis showed that plant GADs are conserved in the process of evolution. The cloning and characterization of the OsGAD3 (SSJ315) gene will enable us to use OsGAD3 to enhance GABA production in O. sativa (SSJ315) by metabolic engineering in the near future.
KeywordCloning Oryza sativa OsGAD3 RT-PCR
DOI10.2298/ABS1004873X
URLView the original
Language英語
Fulltext Access
Citation statistics
Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
AffiliationShanghai Normal University
Recommended Citation
GB/T 7714
Xu H.,Liao P.,Xiao J.,et al. Molecular cloning and characterization of glutamate decarboxylase CDNA from the giant-embryo Oryza sativa[J]. Archives of Biological Sciences,2010,62(4):873-879.
APA Xu H.,Liao P.,Xiao J.,Zhang Q.,Dong Y.,&Kai G..(2010).Molecular cloning and characterization of glutamate decarboxylase CDNA from the giant-embryo Oryza sativa.Archives of Biological Sciences,62(4),873-879.
MLA Xu H.,et al."Molecular cloning and characterization of glutamate decarboxylase CDNA from the giant-embryo Oryza sativa".Archives of Biological Sciences 62.4(2010):873-879.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Xu H.]'s Articles
[Liao P.]'s Articles
[Xiao J.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Xu H.]'s Articles
[Liao P.]'s Articles
[Xiao J.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Xu H.]'s Articles
[Liao P.]'s Articles
[Xiao J.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.