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Increased accumulation of the cardio-cerebrovascular disease treatment drug tanshinone in Salvia miltiorrhiza hairy roots by the enzymes 3-hydroxy-3-methylglutaryl CoA reductase and 1-deoxy-D-xylulose 5-phosphate reductoisomerase
Shi M.3; Luo X.3; Ju G.3; Yu X.2; Hao X.3; Huang Q.3; Xiao J.3; Cui L.3; Kai G.3
2014
Source PublicationFunctional and Integrative Genomics
ISSN14387948 1438793X
Volume14Issue:3Pages:603-615
AbstractTanshinone is widely used for treatment of cardio-cerebrovascular diseases with increasing demand. Herein, key enzyme genes SmHMGR (3-hydroxy-3- methylglutaryl CoA reductase) and SmDXR (1-deoxy-d-xylulose 5-phosphate reductoisomerase) involved in the tanshinone biosynthetic pathway were introduced into Salvia miltiorrhiza (Sm) hairy roots to enhance tanshinone production. Over-expression of SmHMGR or SmDXR in hairy root lines can significantly enhance the yield of tanshinone. Transgenic hairy root lines co-expressing HMGR and DXR (HD lines) produced evidently higher levels of total tanshinone (TT) compared with the control and single gene transformed lines. The highest tanshinone production was observed in HD42 with the concentration of 3.25 mg g DW. Furthermore, the transgenic hairy roots showed higher antioxidant activity than control. In addition, transgenic hairy root harboring HMGR and DXR (HD42) exhibited higher tanshinone content after elicitation by yeast extract and/or Ag than before. Tanshinone can be significantly enhanced to 5.858, 6.716, and 4.426 mg g DW by YE, Ag, and YE-Ag treatment compared with non-induced HD42, respectively. The content of cryptotanshinone and dihydrotanshinone was effectively elevated upon elicitor treatments, whereas there was no obvious promotion effect for the other two compounds tanshinone I and tanshinone IIA. Our results provide a useful strategy to improve tanshinone content as well as other natural active products by combination of genetic engineering with elicitors. © 2014 Springer-Verlag.
KeywordCo-regulation Elicitor Hairy roots Salvia miltiorrhiza Tanshinone
DOI10.1007/s10142-014-0385-0
URLView the original
Language英語
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Cited Times [WOS]:44   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.East China University of Science and Technology
2.Stony Brook University
3.Shanghai Normal University
Recommended Citation
GB/T 7714
Shi M.,Luo X.,Ju G.,et al. Increased accumulation of the cardio-cerebrovascular disease treatment drug tanshinone in Salvia miltiorrhiza hairy roots by the enzymes 3-hydroxy-3-methylglutaryl CoA reductase and 1-deoxy-D-xylulose 5-phosphate reductoisomerase[J]. Functional and Integrative Genomics,2014,14(3):603-615.
APA Shi M..,Luo X..,Ju G..,Yu X..,Hao X..,...&Kai G..(2014).Increased accumulation of the cardio-cerebrovascular disease treatment drug tanshinone in Salvia miltiorrhiza hairy roots by the enzymes 3-hydroxy-3-methylglutaryl CoA reductase and 1-deoxy-D-xylulose 5-phosphate reductoisomerase.Functional and Integrative Genomics,14(3),603-615.
MLA Shi M.,et al."Increased accumulation of the cardio-cerebrovascular disease treatment drug tanshinone in Salvia miltiorrhiza hairy roots by the enzymes 3-hydroxy-3-methylglutaryl CoA reductase and 1-deoxy-D-xylulose 5-phosphate reductoisomerase".Functional and Integrative Genomics 14.3(2014):603-615.
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