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Croton megalobotrys Müll Arg. and Vitex doniana (Sweet): Traditional medicinal plants in a three-step treatment regimen that inhibit in vitro replication of HIV-1
Tietjen I.1; Gatonye T.5; Ngwenya B.N.2; Namushe A.3; Simonambanga S.3; Muzila M.5; Mwimanzi P.1; Xiao J.7; Fedida D.6; Brumme Z.L.1; Brockman M.A.1; Andrae-Marobela K.5
2016-09-15
Source PublicationJournal of Ethnopharmacology
ISSN18727573 03788741
Volume191Pages:331-340
AbstractEthnopharmacological relevance Human Immunodeficiency Virus (HIV) strains resistant to licensed anti-retroviral drugs (ARVs) continue to emerge. On the African continent, uneven access to ARVs combined with occurrence of side-effects after prolonged ARV therapy have led to searches for traditional medicines as alternative or complementary remedies to conventional HIV/AIDS management. Aim of the study Here we characterize a specific three-step traditional HIV/AIDS treatment regimen consisting of Cassia sieberiana root, Vitex doniana root, and Croton megalobotrys bark by combining qualitative interviews of traditional medical knowledge users in Botswana with in vitro HIV replication studies. Materials and methods Crude extracts from a total of seven medicinal plants were tested for in vitro cytotoxicity and inhibition of wild-type (NL4.3) and ARV-resistant HIV-1 replication in an immortalized GFP-reporter CD4+ T-cell line. Results C. sieberiana root, V. doniana root, and C. megalobotrys bark extracts inhibited HIV-1 replication with dose-dependence and without concomitant cytotoxicity. C. sieberiana and V. doniana extracts inhibited HIV-1 replication by 50% at 84.8 µg/mL and at 25 µg/mL, respectively, while C. megalobotrys extracts inhibited HIV-1 replication by a maximum of 45% at concentrations as low as 0.05 µg/mL. Extracts did not interfere with antiviral activities of licensed ARVs when applied in combination and exhibited comparable efficacies against viruses harboring major resistance mutations to licensed protease, reverse-transcriptase, or integrase inhibitors. Conclusions We report for the first time a three-step traditional HIV/AIDS regimen, used alone or in combination with standard ARV regimens, where each step exhibited more potent ability to inhibit HIV replication in vitro. Our observations support the “reverse pharmacology” model where documented clinical experiences are used to identify natural products of therapeutic value.
KeywordAnti-retroviral drugs Botswana Botswana Cassia sieberiana Croton megalobotrys Cytotoxicity HIV-1 Traditional medicine Africa Vitex doniana
DOI10.1016/j.jep.2016.06.040
URLView the original
Language英語
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Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Simon Fraser University
2.Okavango Research Institute
3.Kwame (Legwame) Traditional Association
4.British Columbia Centre for Excellence in HIV-AIDS
5.University of Botswana
6.The University of British Columbia
7.Universidade de Macau
Recommended Citation
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Tietjen I.,Gatonye T.,Ngwenya B.N.,et al. Croton megalobotrys Müll Arg. and Vitex doniana (Sweet): Traditional medicinal plants in a three-step treatment regimen that inhibit in vitro replication of HIV-1[J]. Journal of Ethnopharmacology,2016,191:331-340.
APA Tietjen I..,Gatonye T..,Ngwenya B.N..,Namushe A..,Simonambanga S..,...&Andrae-Marobela K..(2016).Croton megalobotrys Müll Arg. and Vitex doniana (Sweet): Traditional medicinal plants in a three-step treatment regimen that inhibit in vitro replication of HIV-1.Journal of Ethnopharmacology,191,331-340.
MLA Tietjen I.,et al."Croton megalobotrys Müll Arg. and Vitex doniana (Sweet): Traditional medicinal plants in a three-step treatment regimen that inhibit in vitro replication of HIV-1".Journal of Ethnopharmacology 191(2016):331-340.
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