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Separation of oligosaccharides from lotus seeds via medium-pressure liquid chromatography coupled with ELSD and DAD
Lu X.1; Zheng Z.1; Miao S.2; Li H.1; Guo Z.1; Zhang Y.1; Zheng Y.1; Zheng B.1; Xiao J.1
2017-03-09
Source PublicationScientific Reports
ISSN20452322
Volume7
Abstract

Lotus seeds were identified by the Ministry of Public Health of China as both food and medicine. One general function of lotus seeds is to improve intestinal health. However, to date, studies evaluating the relationship between bioactive compounds in lotus seeds and the physiological activity of the intestine are limited. In the present study, by using medium pressure liquid chromatography coupled with evaporative light-scattering detector and diode-array detector, five oligosaccharides were isolated and their structures were further characterized by electrospray ionization-mass spectrometry and gas chromatography-mass spectrometry. In vitro testing determined that LOS3-1 and LOS4 elicited relatively good proliferative effects on Lactobacillus delbrueckii subsp. bulgaricus. These results indicated a structure-function relationship between the physiological activity of oligosaccharides in lotus seeds and the number of probiotics applied, thus providing room for improvement of this particular feature. Intestinal probiotics may potentially become a new effective drug target for the regulation of immunity.

DOI10.1038/srep44174
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000395974700001
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Cited Times [WOS]:4   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Fujian Agriculture and Forestry University
2.Fermoy, Co. Cork
3.Universidade de Macau
Recommended Citation
GB/T 7714
Lu X.,Zheng Z.,Miao S.,et al. Separation of oligosaccharides from lotus seeds via medium-pressure liquid chromatography coupled with ELSD and DAD[J]. Scientific Reports,2017,7.
APA Lu X..,Zheng Z..,Miao S..,Li H..,Guo Z..,...&Xiao J..(2017).Separation of oligosaccharides from lotus seeds via medium-pressure liquid chromatography coupled with ELSD and DAD.Scientific Reports,7.
MLA Lu X.,et al."Separation of oligosaccharides from lotus seeds via medium-pressure liquid chromatography coupled with ELSD and DAD".Scientific Reports 7(2017).
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