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Hepatoprotective activity of Ganoderma lucidum triterpenoids in alcohol-induced liver injury in mice, an iTRAQ-based proteomic analysis
Zhao C.1; Fan J.2,3; Liu Y.1; Guo W.1; Cao H.4; Xiao J.4; Wang Y.4; Liu B.1,3
2019-01-15
Source PublicationFood Chemistry
ISSN18737072 03088146
Volume271Pages:148-156
Abstract

The hepatoprotective activity of ethanol extract of Ganoderma lucidum (GLE) in alcohol-induced liver injury in mice was investigated. HPLC coupled with photo-diode array detector and electrospray ionization-mass spectrometry was used to analyze the major triterpenoids in GLE. The effects of GLE on hepatoprotection were evaluated through histopathology and biochemical analysis of serum enzymes. We used isobaric tag for relative and absolute quantitation (iTRAQ) coupled with tandem mass spectrometry to identify differentially expressed liver proteome in mice. There were more than 4000 differentially expressed proteins; 40 proteins with the most significant changed proteins were applied for further bioinformatics analysis. Expression levels of cytochrome P450 2E1 and alcohol dehydrogenase 1, proteins that are closely associated with these processes, were validated by western blotting. Triterpenoids, major components of GLE, protected alcohol-induced liver injury through inhibiting lipid peroxidation, elevating activity of antioxidant enzymes, and suppressing apoptotic cell death and immune inflammatory response.

KeywordAlcohol-induced Liver Injury Ganoderma Lucidum Hepatoprotection Itraq Triterpenoids
DOI10.1016/j.foodchem.2018.07.115
URLView the original
Indexed BySCIE
WOS Research AreaChemistry ; Nutrition & Dietetics ; Food Science & Technology
WOS SubjectChemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
WOS IDWOS:000444967800020
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Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
2.College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
3.National Engineering Research Center of JUNCAO, Fuzhou 350002, China
4.Institute of Chinese Medical Sciences and State Key Laboratory of Quality Control in Chinese Medicine, University of Macau, Macau
Recommended Citation
GB/T 7714
Zhao C.,Fan J.,Liu Y.,et al. Hepatoprotective activity of Ganoderma lucidum triterpenoids in alcohol-induced liver injury in mice, an iTRAQ-based proteomic analysis[J]. Food Chemistry,2019,271:148-156.
APA Zhao C..,Fan J..,Liu Y..,Guo W..,Cao H..,...&Liu B..(2019).Hepatoprotective activity of Ganoderma lucidum triterpenoids in alcohol-induced liver injury in mice, an iTRAQ-based proteomic analysis.Food Chemistry,271,148-156.
MLA Zhao C.,et al."Hepatoprotective activity of Ganoderma lucidum triterpenoids in alcohol-induced liver injury in mice, an iTRAQ-based proteomic analysis".Food Chemistry 271(2019):148-156.
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