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Hepatoprotective activity of Ganoderma lucidum triterpenoids in alcohol-induced liver injury in mice, an iTRAQ-based proteomic analysis
Zhao C.1; Fan J.1; Liu Y.1; Guo W.1; Cao H.3; Xiao J.3; Wang Y.3; Liu B.1
2019-01-15
Source PublicationFood Chemistry
ISSN18737072 03088146
Volume271Pages:148-156
AbstractThe hepatoprotective activity of ethanol extract of Ganoderma lucidum (GLE) in alcohol-induced liver injury in mice was investigated. HPLC coupled with photo-diode array detector and electrospray ionization-mass spectrometry was used to analyze the major triterpenoids in GLE. The effects of GLE on hepatoprotection were evaluated through histopathology and biochemical analysis of serum enzymes. We used isobaric tag for relative and absolute quantitation (iTRAQ) coupled with tandem mass spectrometry to identify differentially expressed liver proteome in mice. There were more than 4000 differentially expressed proteins; 40 proteins with the most significant changed proteins were applied for further bioinformatics analysis. Expression levels of cytochrome P450 2E1 and alcohol dehydrogenase 1, proteins that are closely associated with these processes, were validated by western blotting. Triterpenoids, major components of GLE, protected alcohol-induced liver injury through inhibiting lipid peroxidation, elevating activity of antioxidant enzymes, and suppressing apoptotic cell death and immune inflammatory response.
KeywordAlcohol-induced liver injury Ganoderma lucidum Hepatoprotection iTRAQ Triterpenoids
DOI10.1016/j.foodchem.2018.07.115
URLView the original
Language英語
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Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Fujian Agriculture and Forestry University
2.National Engineering Research Center of Juncao Technology
3.Universidade de Macau
Recommended Citation
GB/T 7714
Zhao C.,Fan J.,Liu Y.,et al. Hepatoprotective activity of Ganoderma lucidum triterpenoids in alcohol-induced liver injury in mice, an iTRAQ-based proteomic analysis[J]. Food Chemistry,2019,271:148-156.
APA Zhao C..,Fan J..,Liu Y..,Guo W..,Cao H..,...&Liu B..(2019).Hepatoprotective activity of Ganoderma lucidum triterpenoids in alcohol-induced liver injury in mice, an iTRAQ-based proteomic analysis.Food Chemistry,271,148-156.
MLA Zhao C.,et al."Hepatoprotective activity of Ganoderma lucidum triterpenoids in alcohol-induced liver injury in mice, an iTRAQ-based proteomic analysis".Food Chemistry 271(2019):148-156.
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