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Hydrotalcite intercalated siRNA: Computational characterization of the interlayer environment
Zhang H.1; Ouyang D.5; Murthy V.3; Wong Y.2; Xu Z.1; Smith S.C.4
2012-06-01
Source PublicationPharmaceutics
ISSN19994923
Volume4Issue:2Pages:296-313
AbstractUsing molecular dynamics (MD) simulations, we explore the structural and dynamical properties of siRNA within the intercalated environment of a Mg:Al 2:1 Layered Double Hydroxide (LDH) nanoparticle. An ab initio force field (Condensed-phase Optimized Molecular Potentials for Atomistic Simulation Studies: COMPASS) is used for the MD simulations of the hybrid organic-inorganic systems. The structure, arrangement, mobility, close contacts and hydrogen bonds associated with the intercalated RNA are examined and contrasted with those of the isolated RNA. Computed powder X-ray diffraction patterns are also compared with related LDH-DNA experiments. As a method ofprobing whether the intercalated environment approximates the crystalline or rather the aqueous state, we explore the stability of the principle parameters (e.g., the major groove width) that differentiate both A- and A'- crystalline forms of siRNA and contrast this with recent findings for the same siRNA simulated in water. We find the crystalline forms remain structurally distinct when intercalated, whereas this is not the case in water. Implications for the stability of hybrid LDH-RNA systems are discussed. © 2012 by the authors; licensee MDPI, Basel, Switzerland.
KeywordGene therapy Layered double hydroxide Molecular dynamics simulations SiRNA delivery
DOI10.3390/pharmaceutics4020296
URLView the original
Language英語
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Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.University of Queensland
2.Singapore Polytechnic
3.Charles Darwin University
4.Oak Ridge National Laboratory
5.Aston University
Recommended Citation
GB/T 7714
Zhang H.,Ouyang D.,Murthy V.,et al. Hydrotalcite intercalated siRNA: Computational characterization of the interlayer environment[J]. Pharmaceutics,2012,4(2):296-313.
APA Zhang H.,Ouyang D.,Murthy V.,Wong Y.,Xu Z.,&Smith S.C..(2012).Hydrotalcite intercalated siRNA: Computational characterization of the interlayer environment.Pharmaceutics,4(2),296-313.
MLA Zhang H.,et al."Hydrotalcite intercalated siRNA: Computational characterization of the interlayer environment".Pharmaceutics 4.2(2012):296-313.
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