UM  > 中華醫藥研究院
Regulation of skeletal muscle oxidative capacity and muscle mass by SIRT3
Lin L.1; Chen K.1; Khalek W.A.3; Ward J.L.1; Yang H.1; Chabi B.3; Wrutniak-Cabello C.3; Tong Q.1
Source PublicationPLoS ONE

We have previously reported that the expression of mitochondrial deacetylase SIRT3 is high in the slow oxidative muscle and that the expression of muscle SIRT3 level is increased by dietary restriction or exercise training. To explore the function of SIRT3 in skeletal muscle, we report here the establishment of a transgenic mouse model with muscle-specific expression of the murine SIRT3 short isoform (SIRT3M3). Calorimetry study revealed that the transgenic mice had increased energy expenditure and lower respiratory exchange rate (RER), indicating a shift towards lipid oxidation for fuel usage, compared to control mice. The transgenic mice exhibited better exercise performance on treadmills, running 45% further than control animals. Moreover, the transgenic mice displayed higher proportion of slow oxidative muscle fibers, with increased muscle AMPK activation and PPARd expression, both of which are known regulators promoting type I muscle fiber specification. Surprisingly, transgenic expression of SIRT3M3 reduced muscle mass up to 30%, likely through an up-regulation of FOXO1 transcription factor and its downstream atrophy gene MuRF-1. In summary, these results suggest that SIRT3 regulates the formation of oxidative muscle fiber, improves muscle metabolic function, and reduces muscle mass, changes that mimic the effects of caloric restriction. © 2014 Lin et al.

URLView the original
Indexed BySCI
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000330235100111
Fulltext Access
Citation statistics
Cited Times [WOS]:25   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Baylor College of Medicine
2.Dynamique Musculaire et Métabolisme
3.Universidade de Macau
Recommended Citation
GB/T 7714
Lin L.,Chen K.,Khalek W.A.,et al. Regulation of skeletal muscle oxidative capacity and muscle mass by SIRT3[J]. PLoS ONE,2014,9(1).
APA Lin L..,Chen K..,Khalek W.A..,Ward J.L..,Yang H..,...&Tong Q..(2014).Regulation of skeletal muscle oxidative capacity and muscle mass by SIRT3.PLoS ONE,9(1).
MLA Lin L.,et al."Regulation of skeletal muscle oxidative capacity and muscle mass by SIRT3".PLoS ONE 9.1(2014).
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Lin L.]'s Articles
[Chen K.]'s Articles
[Khalek W.A.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Lin L.]'s Articles
[Chen K.]'s Articles
[Khalek W.A.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Lin L.]'s Articles
[Chen K.]'s Articles
[Khalek W.A.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.