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Personalized chemotherapy profiling using cancer cell lines from selectable mice
Kamiyama H.1; Rauenzahn S.1; Shim J.S.1; Karikari C.A.1; Feldmann G.1; Hua L.1; Kamiyama M.1; Schuler F.W.1; Lin M.-T.1; Beaty R.M.1; Karanam B.1; Liang H.1; Mullendore M.E.1; Mo G.1; Hidalgo M.1; Jaffee E.1; Hruban R.H.1; Jinnah H.A.2; Roden R.B.S.1; Jimeno A.1; Liu J.O.1; Maitra A.1; Eshleman J.R.1
2013-03-01
Source PublicationClinical Cancer Research
ISSN10780432 15573265
Volume19Issue:5Pages:1139-1146
Abstract

Purpose: High-throughput chemosensitivity testing of low-passage cancer cell lines can be used to prioritize agents for personalized chemotherapy. However, generating cell lines from primary cancers is difficult because contaminating stromal cells overgrow the malignant cells. Experimental Design: We produced a series of hypoxanthine phosphoribosyl transferase (hprt)-null immunodeficient mice. During growth of human cancers in these mice, hprt-null murine stromal cells replace their human counterparts. Results: Pancreatic and ovarian cancers explanted from these mice were grown in selection media to produce pure human cancer cell lines. Wescreened one cell line with a 3,131-drug panel and identified 77 U.S. Food and Drug Administration (FDA)-approved drugs with activity, and two novel drugs to which the cell line was uniquely sensitive. Xenografts of this carcinoma were selectively responsive to both drugs. Conclusion: Chemotherapy can be personalized using patient-specific cell lines derived in biochemically selectable mice. © 2012 American Association for Cancer Research.

DOI10.1158/1078-0432.CCR-12-2127
URLView the original
Indexed BySCI
Language英语
WOS Research AreaOncology
WOS SubjectOncology
WOS IDWOS:000315740200021
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Cited Times [WOS]:19   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.The Johns Hopkins School of Medicine
2.Emory University School of Medicine
Recommended Citation
GB/T 7714
Kamiyama H.,Rauenzahn S.,Shim J.S.,et al. Personalized chemotherapy profiling using cancer cell lines from selectable mice[J]. Clinical Cancer Research,2013,19(5):1139-1146.
APA Kamiyama H..,Rauenzahn S..,Shim J.S..,Karikari C.A..,Feldmann G..,...&Eshleman J.R..(2013).Personalized chemotherapy profiling using cancer cell lines from selectable mice.Clinical Cancer Research,19(5),1139-1146.
MLA Kamiyama H.,et al."Personalized chemotherapy profiling using cancer cell lines from selectable mice".Clinical Cancer Research 19.5(2013):1139-1146.
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