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Protection of Total Flavonoid Fraction from Nervilia fordii on Lipopolysaccharide-induced Acute Lung Injury in Rats
HUANG Ming-qing1; XIE You-liang2; LAI Xiao-ping2; LIN Ling3; XU Yin-ji3; LU Jin-jian4; CHEN Xiu-ping4
2012
Source PublicationChinese Herbal Medicines
ISSN2589-3610
Volume4Issue:4Pages:287-293
Abstract

Objective

To investigate the effects of total flavonoid fraction (TFF) from Nervilia fordii on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats, and to explore their protective mechanism.

Methods

LPS-induced ALI model was established by LPS (5 mg/kg) injection via left cervical vein. Blood samples were collected from the cervical artery of all rats at 5 and 6 h after LPS challenge for arterial blood gas test and cytokines measurements, and pulmonary microvascular permeability (PMP), lung wet/dry weight ratio (W/D), and pathological features were observed.

Results

Phytochemical study showed that the TFF contained 67.3% of flavonoids expressed in rutin and three flavone glycosides. The TFF pretreatment (6.24 and 12.48 mg/kg) attenuated the partial arterial pressure of oxygen decline in blood significantly, and decreased the PMP and lung W/D in ALI rats. In addition, the TFF (6.24 and 12.48 mg/kg) also ameliorated the LPS-induced lung damages including alveolar edema, neutrophils infiltration, alveolar hemorrhage, and thickening of the alveolar wall. Furthermore, the treatment with the TFF (6.24 and 12.48 mg/kg) also down-regulated the levels of pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and intercellular adhesion molecule-1 (ICAM-1), and up-regulated the level of anti-inflammatory cytokine IL-10 in serum of ALI rats simultaneously.

Conclusion

These results suggest that the TFF could protect LPS-induced ALI in rats, which may be mediated, at least in part, by adjusting the production of inflammatory cytokines including TNF-α, IL-6, ICAM-1, and IL-10.

KeywordAcute Lung Injury Cytokine Nervilia Fordii Pulmonary Microvascular Permeability Total Flavonoid Fraction
DOIhttps://doi.org/10.3969/j.issn.1674-6348.2012.04.005
Language英语
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Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China
2.Institute of Drug Research and Development, Guangzhou University of Chinese Medicine, Guangzhou 510405, China
3.Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510120, China
4.State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Macao 999078, China
Recommended Citation
GB/T 7714
HUANG Ming-qing,XIE You-liang,LAI Xiao-ping,et al. Protection of Total Flavonoid Fraction from Nervilia fordii on Lipopolysaccharide-induced Acute Lung Injury in Rats[J]. Chinese Herbal Medicines,2012,4(4):287-293.
APA HUANG Ming-qing.,XIE You-liang.,LAI Xiao-ping.,LIN Ling.,XU Yin-ji.,...&CHEN Xiu-ping.(2012).Protection of Total Flavonoid Fraction from Nervilia fordii on Lipopolysaccharide-induced Acute Lung Injury in Rats.Chinese Herbal Medicines,4(4),287-293.
MLA HUANG Ming-qing,et al."Protection of Total Flavonoid Fraction from Nervilia fordii on Lipopolysaccharide-induced Acute Lung Injury in Rats".Chinese Herbal Medicines 4.4(2012):287-293.
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