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Selective imaging in second-harmonic-generation microscopy with anisotropic radiation
Shi-Wei Chu1; Shih-Peng Tai2; Tzu-Ming Liu2; Chi-Kuang Sun2; Chi-Hung Linc3
2009
Source PublicationJournal of Biomedical Optics
ISSN1083-3668
Volume14Issue:1
Abstract

As a novel modality of optical microscopy, second-harmonic generation SHG provides attractive features including intrinsic optical sectioning, noninvasiveness, high specificity, and high penetrability. For a biomedical application, the epicollection of backward propagating SHG is necessary. But due to phase-matching constraint, SHG from thick tissues is preferentially forward propagation. Myosin and collagen are two of the most abundant fibrous proteins in vertebrates, and both exhibit a strong second-harmonic response. We find that the radiation patterns of myosin-based muscle fibers and collagen fibrils are distinct due to coherence effects. Based on these asymmetric radiation patterns, we demonstrate selective imaging between intertwining muscle fibers and type I collagen fibrils with forward and backward SHG modalities, respectively. Thick muscle fibers dominate the forward signal, while collagen fibril distribution is preferentially resolved in the backward channel without strong interference from muscle. Moreover, we find that wellformed collagen fibrils are highlighted by forward SHG, while loosely arranged collagen matrix is outlined by backward signal.

KeywordMicroscopy Nonlinear Optics Anisotropy Issues Biomedical Optics Second-harmonic Generation
DOIhttp://dx.doi.org/10.1117/1.3080722
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS SubjectBiochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS IDWOS:000264551900003
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Cited Times [WOS]:14   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Department of Physics, National Taiwan University, Taipei 10617, Taiwan
2.Graduate Institute of Photonics and Optoelectronics and Department of Electrical Engineering, National Taiwan University, Taipei 10617, Taiwan
3.Institute of Microbiology and Immunology, National Yang-Ming University, Taipei 11221, Taiwan
Recommended Citation
GB/T 7714
Shi-Wei Chu,Shih-Peng Tai,Tzu-Ming Liu,et al. Selective imaging in second-harmonic-generation microscopy with anisotropic radiation[J]. Journal of Biomedical Optics,2009,14(1).
APA Shi-Wei Chu,Shih-Peng Tai,Tzu-Ming Liu,Chi-Kuang Sun,&Chi-Hung Linc.(2009).Selective imaging in second-harmonic-generation microscopy with anisotropic radiation.Journal of Biomedical Optics,14(1).
MLA Shi-Wei Chu,et al."Selective imaging in second-harmonic-generation microscopy with anisotropic radiation".Journal of Biomedical Optics 14.1(2009).
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