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Preparative separation of minor saponins from Panax notoginseng leaves using biotransformation, macroporous resins, and preparative high-performance liquid chromatography
Fang Liu1; Ni Ma2; Fang-Bo Xia1; Peng Li1; Chengwei He1; Zhenqiang Wu3; Jian-Bo Wan1,4
2017-10-16
Source PublicationJournal of Ginseng Research
ISSN1226-8453
Volume43Issue:1Pages:105-115
Abstract

Background

Ginsenosides with less sugar moieties may exhibit the better adsorptive capacity and more pharmacological activities.

Methods

An efficient method for the separation of four minor saponins, including gypenoside XVII, notoginsenoside Fe, ginsenoside Rd2, and notoginsenoside Fd, from Panax notoginseng leaves (PNL) was established using biotransformation, macroporous resins, and subsequent preparative high-performance liquid chromatography.

Results

The dried PNL powder was immersed in the distilled water at 50°C for 30 min for converting the major saponins, ginsenosides Rb1, Rc, Rb2, and Rb3, to minor saponins, gypenoside XVII, notoginsenoside Fe, ginsenoside Rd2, and notoginsenoside Fd, respectively, by the enzymes present in PNL. The adsorption characteristics of these minor saponins on five types of macroporous resins, D-101, DA-201, DM-301, X-5, and S-8, were evaluated and compared. Among them, D-101 was selected due to the best adsorption and desorption properties. Under the optimized conditions, the fraction containing the four target saponins was separated by D-101 resin. Subsequently, the target minor saponins were individually separated and purified by preparative high-performance liquid chromatography with a reversed-phase column.

Conclusion

Our study provides a simple and efficient method for the preparation of these four minor saponins from PNL, which will be potential for industrial applications.

KeywordGinsenoside Rd2 Gypenoside Xvii Notoginsenoside Fd Notoginsenoside Fe Panax Notoginseng Leaves
DOIhttps://doi.org/10.1016/j.jgr.2017.09.003
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS SubjectPlant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine
WOS IDWOS:000454927200012
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Citation statistics
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorJian-Bo Wan
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China
2.Department of Product Development, Wenshan Sanqi Institute of Science and Technology, Wenshan University, Wenshan, Yunnan, China
3.School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, China
4.Zhuhai UM Science & Technology Research Institute, Zhuhai, Guangdong, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Fang Liu,Ni Ma,Fang-Bo Xia,et al. Preparative separation of minor saponins from Panax notoginseng leaves using biotransformation, macroporous resins, and preparative high-performance liquid chromatography[J]. Journal of Ginseng Research,2017,43(1):105-115.
APA Fang Liu.,Ni Ma.,Fang-Bo Xia.,Peng Li.,Chengwei He.,...&Jian-Bo Wan.(2017).Preparative separation of minor saponins from Panax notoginseng leaves using biotransformation, macroporous resins, and preparative high-performance liquid chromatography.Journal of Ginseng Research,43(1),105-115.
MLA Fang Liu,et al."Preparative separation of minor saponins from Panax notoginseng leaves using biotransformation, macroporous resins, and preparative high-performance liquid chromatography".Journal of Ginseng Research 43.1(2017):105-115.
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