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Virtual Optical Biopsy of Human Adipocytes with third harmonic generation microscopy
Tsai, CK1; Wang, TD2,3,4,5,6; Lin, JW2,3; Hsu, RB7; Guo, LZ1; Chen, ST1; Liu, TM1,6
2013
Source PublicationBIOMEDICAL OPTICS EXPRESS
ISSN2156-7085
Volume4Issue:1Pages:178-186
Abstract

Using the sectioning capability of third harmonic generation (THG) microscopy, we assessed the morphologic features of human adipocytes directly without fixation and labeling. At the plane of the largest cross-sectional area, both area-equivalent circular diameters (AECD) and perimeters ofadipocytes were measured, and their statistical distributions were examined. We found, in patients with no cardiovascular risk factors, the average AECD of epicardial adipocytes were 70-90 mu m with11-17 mu m standard deviations. In contrast, for patients with coronary artery disease, amounts ofsmall-sized (AECD <40 mu m) epicardial adipocytes were observed and the corresponding standard deviations of AECD were increased to 20-29 mu m. Our results indicate that the THG tomography platform can be used to explore the histopathological features of adipocytes in clinical scenarios based on its superior resolution for virtual optical biopsy. (C) 2012 Optical Society of America

DOIhttp://doi.org/10.1364/BOE.4.000178
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS SubjectBiochemical Research Methods ; Optics ; Radiology, Nuclear Medicine & Medical Imaging
WOS IDWOS:000312710100015
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Cited Times [WOS]:14   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Natl Taiwan Univ, Inst Biomed Engn, Taipei 10617, Taiwan
2.Natl Taiwan Univ, Coll Med, Taipei 10617, Taiwan
3.Natl Taiwan Univ Hosp, Div Cardiol, Dept Internal Med, Taipei 10617, Taiwan
4.Natl Taiwan Univ Hosp, Yun Lin Branch, Dept Internal Med, Douliu City 64041, Yun Lin County, Taiwan
5.Natl Taiwan Univ Hosp, Cardiovasc Ctr, Douliu City 64041, Yun Lin County, Taiwan
6.Natl Taiwan Univ, Mol Imaging Ctr, Taipei 10617, Taiwan
7.Natl Taiwan Univ Hosp, Dept Surg, Taipei 10048, Taiwan
Recommended Citation
GB/T 7714
Tsai, CK,Wang, TD,Lin, JW,et al. Virtual Optical Biopsy of Human Adipocytes with third harmonic generation microscopy[J]. BIOMEDICAL OPTICS EXPRESS,2013,4(1):178-186.
APA Tsai, CK.,Wang, TD.,Lin, JW.,Hsu, RB.,Guo, LZ.,...&Liu, TM.(2013).Virtual Optical Biopsy of Human Adipocytes with third harmonic generation microscopy.BIOMEDICAL OPTICS EXPRESS,4(1),178-186.
MLA Tsai, CK,et al."Virtual Optical Biopsy of Human Adipocytes with third harmonic generation microscopy".BIOMEDICAL OPTICS EXPRESS 4.1(2013):178-186.
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