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In vivo developmental biology study using noninvasive multi-harmonic generation microscopy
Shi-Wei Chu1; Szu-Yu Chen1; Tsung-Han Tsai1; Tzu-Ming Liu1; Cheng-Yung Lin2; Huai-Jen Tsai2; Chi-Kuang Sun3
2003
Source PublicationOPTICS EXPRESS
ISSN1094-4087
Volume11Issue:23Pages:3093-3099
Abstract

Morphological changes and complex developmental processes inside vertebrate embryos are difficult to observe noninvasively with millimeter-penetration and sub-micrometer-resolution at the same time. By using higher harmonic generation, including second and third harmonics, as the microscopic contrast mechanism, optical noninvasiveness can be achieved due to the virtual-level-transition characteristic. The intrinsic nonlinearity of harmonic generations provides optical sectioning capability while the selected 1230-nm near-infrared light source provides the deep-penetration ability. The complicated development within a similar to 1.5-mm thick zebrafish (Danio rerio) embryo from initial cell proliferation, gastrulation, to tissue formation can all be observed clearly in vivowithout any treatment on the live specimen. (C) 2003 Optical Society of America.

DOIhttps://doi.org/10.1364/OE.11.003093
Indexed BySCI
Language英语
WOS Research AreaOptics
WOS SubjectOptics
WOS IDWOS:000186641100012
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Cited Times [WOS]:131   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Department of Electrical Engineering and Graduate Institute of Electro-Optical Engineering, National Taiwan University, Taipei, 10617 Taiwan, R.O.C.
2.Institute of Molecular and Cell Biology, National Taiwan University, Taipei 10617, Taiwan, R.O.C.
3.Department of Electrical Engineering and Graduate Institute of Electro-Optical Engineering, National Taiwan University, Taipei, 10617 Taiwan, R.O.C.
Recommended Citation
GB/T 7714
Shi-Wei Chu,Szu-Yu Chen,Tsung-Han Tsai,et al. In vivo developmental biology study using noninvasive multi-harmonic generation microscopy[J]. OPTICS EXPRESS,2003,11(23):3093-3099.
APA Shi-Wei Chu.,Szu-Yu Chen.,Tsung-Han Tsai.,Tzu-Ming Liu.,Cheng-Yung Lin.,...&Chi-Kuang Sun.(2003).In vivo developmental biology study using noninvasive multi-harmonic generation microscopy.OPTICS EXPRESS,11(23),3093-3099.
MLA Shi-Wei Chu,et al."In vivo developmental biology study using noninvasive multi-harmonic generation microscopy".OPTICS EXPRESS 11.23(2003):3093-3099.
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