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Quantitative and qualitative investigation into the impact of focused ultrasound with microbubbles on the triggered release of nanoparticles from vasculature in mouse tumors
Lin, CY1,2; Liu, TM1; Chen, CY3; Huang, YL4; Huang, WK3; Sun, CK5,6; Chang, FH3; Lin, WL1,2
2010
Source PublicationJOURNAL OF CONTROLLED RELEASE
ISSN0168-3659
Volume146Issue:3Pages:291-298
Abstract

Ultrasound-mediated microbubble destruction may enhance the release of nanoparticles fromvasculature to tumor tissues. In this study, we used four different sizes of lipid-coated CdSe quantum dot (LQD) nanoparticles ranging from 30 to 180 nm, 1.0-MHz pulsed focused ultrasound (FUS) with a peak acoustic pressure of 1.2-MPa, and an ultrasound contrast agent (UCA; SonoVue (R)) at a dose of30 mu L/kg to investigate any enhancement of targeted delivery. Tumor-bearing male Balb/c mice were first injected with UCA intravenously, were then sonicated at the tumors with FUS, and were finally injected with 50 mu L. of the LQD solution after the sonication. The mice were sacrificed about 24 h after the sonication, and then we quantitatively and qualitatively evaluated the deposition ofLQDs in the tumors by using graphite furnace atomic absorption spectrometry (GF-AAS), photoluminescence spectrometry (PL), and harmonic generation microscopy (HGM). Further, immunoblotting analysis served to identify the biochemical markers reflecting the vascular rupture. The experimental results show that the amount of LQDs deposited in tumor tissues was greater incases of FUS/UCA application, especially for smaller LQDs, being 4.47, 2.27, 0.99, and 0.82 (mu g Cd)/(g tumor) for 30, 80, 130, and 180 nm of LQDs, respectively; compared to 1.12, 0.75, 0.26, and 0.34 (mu g Cd)/ (g tumor) in absence of FUS/UCA. The immunoblotting analysis further indicates that FUS-induced UCA oscillation/destruction results in rupture areas in blood vessels increasing the vascular permeability and thus justifying for the higher quantity of nanoparticles deposited in tumors. (c) 2010 Elsevier BM. All rights reserved.

KeywordFocused Ultrasound Microbubbles Lipid-coated Quantum Dots Nanoparticle Delivery Tumor
DOIhttp://dx.doi.org/10.1016/j.jconrel.2010.05.033
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS SubjectChemistry, Multidisciplinary ; Pharmacology & Pharmacy
WOS IDWOS:000282398100004
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Cited Times [WOS]:51   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Natl Taiwan Univ, Inst Biomed Engn, Taipei 100, Taiwan
2.Natl Hlth Res Inst, Div Med Engn Res, Miaoli, Taiwan
3.Natl Taiwan Univ, Inst Biochem & Mol Biol, Taipei 100, Taiwan
4.hang Gung Mem Hosp, Dept Nephrol, Tao Yuan, Taiwan
5.Natl Taiwan Univ, Dept Elect Engn, Taipei 100, Taiwan
6.Natl Taiwan Univ, Grad Inst Photon & Optoelect, Taipei 100, Taiwan
Recommended Citation
GB/T 7714
Lin, CY,Liu, TM,Chen, CY,et al. Quantitative and qualitative investigation into the impact of focused ultrasound with microbubbles on the triggered release of nanoparticles from vasculature in mouse tumors[J]. JOURNAL OF CONTROLLED RELEASE,2010,146(3):291-298.
APA Lin, CY.,Liu, TM.,Chen, CY.,Huang, YL.,Huang, WK.,...&Lin, WL.(2010).Quantitative and qualitative investigation into the impact of focused ultrasound with microbubbles on the triggered release of nanoparticles from vasculature in mouse tumors.JOURNAL OF CONTROLLED RELEASE,146(3),291-298.
MLA Lin, CY,et al."Quantitative and qualitative investigation into the impact of focused ultrasound with microbubbles on the triggered release of nanoparticles from vasculature in mouse tumors".JOURNAL OF CONTROLLED RELEASE 146.3(2010):291-298.
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