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Quantitative determination of arenobufagin in rat plasma by ultra fast liquid chromatography-tandem mass spectrometry and its application in a pharmacokinetic study
Li G.1; Han W.1; Jiang W.1; Zhang D.2; Ye W.2; Chen X.3; Ma A.1
2013-11-15
Source PublicationJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ISSN15700232 1873376X
Volume939Pages:86-91
Abstract

A rapid, sensitive, and selective ultra fast liquid chromatography-tandem mass spectrometry method was developed for quantitative determination of arenobufagin in rat plasma. Sample pretreatment involved a one-step protein precipitation with methanol using 0.1mL rat plasma. The separation was carried out on a Shim-pack XR-ODS II (75mm×2.0mm, i.d. 2.1μm) column with gradient elution at a flow rate of 0.30mLmin. The mobile phase was acetonitrile and 0.1% formic acid in water. A post-column switching valve was applied to reduce the matrix effect. The detection was performed on a triple-quadruple tandem mass spectrometer in the multiple reaction monitoring mode after electrospray ionization. Linear calibration curves for arenobufagin were obtained over the concentration range 1.056-1056ngmL, with a lower limit of quantification of 1.056ngmL. The intra-day and inter-day precision values were lower than 15% and the accuracy ranged from 5.4% to 9.8% at all quality control levels. The method was successfully applied to the determination and pharmacokinetic study of arenobufagin in rat plasma following intraperitoneal administration. © 2013 Elsevier B.V.

KeywordArenobufagin Intraperitoneal Administration Pharmacokinetics Rat Plasma Uflc-esi-ms/ms
DOI10.1016/j.jchromb.2013.09.019
URLView the original
Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000326010400013
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Cited Times [WOS]:6   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Southern Medical University
2.Jinan University
3.Universidade de Macau
Recommended Citation
GB/T 7714
Li G.,Han W.,Jiang W.,et al. Quantitative determination of arenobufagin in rat plasma by ultra fast liquid chromatography-tandem mass spectrometry and its application in a pharmacokinetic study[J]. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences,2013,939:86-91.
APA Li G..,Han W..,Jiang W..,Zhang D..,Ye W..,...&Ma A..(2013).Quantitative determination of arenobufagin in rat plasma by ultra fast liquid chromatography-tandem mass spectrometry and its application in a pharmacokinetic study.Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences,939,86-91.
MLA Li G.,et al."Quantitative determination of arenobufagin in rat plasma by ultra fast liquid chromatography-tandem mass spectrometry and its application in a pharmacokinetic study".Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 939(2013):86-91.
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