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Synergistic delivery of gold nanorods using multifunctional microbubbles for enhanced plasmonic photothermal therapy
Yu-Hsin Wang1; Shi-Ping Chen1; Ai-Ho Liao2; Ya-Chuen Yang3; Cheng-Ru Lee3; Cheng-Han Wu4; Pei-Chun Wu4; Tzu-Ming Liu4; Churng-Ren Chris Wang3; Pai-Chi Li1,5
2014-07-14
Source PublicationScientific Reports
ISSN2045-2322
Volume4Pages:5685
Abstract

Plasmonic photothermal therapy (PPTT) using plasmonic nanoparticles as efficient photoabsorbing agents has been proposed previously. One critical step in PPTT is to effectively deliver gold nanoparticles into the cells. This study demonstrates that the delivery of gold nanorods (AuNRs) can be greatly enhanced by combining the following three mechanisms: AuNRs encapsulated in protein-shell microbubbles (AuMBs), molecular targeting, and sonoporation employing acoustic cavitation of microbubbles (MBs). Both in vitro and in vivo tests were performed. For molecular targeting, the AuMBs were modified with anti-VEGFR2. Once bound to the angiogenesis markers, the MBs were destroyed by ultrasound to release the AuNRs and the release was confirmed by photoacoustic measurements. Additionally, acoustic cavitation was induced during MB destruction for sonoporation (i.e., increase in transient cellular permeability). The measured inertial cavitation dose was positively correlated with the temperature increase at the tumor site. The quantity of AuNRs delivered into the cells was also determined by measuring the mass spectrometry and observed using third-harmonic-generation microscopy and two-photon fluorescence microscopy. A temperature increase of 20°C was achieved in vitro. The PPTT results in vivo also demonstrated that the temperature increase (>45°C) provided a sufficiently high degree of hyperthermia. Therefore, synergistic delivery of AuNRs was demonstrated.

DOIhttp://doi.org/10.1038/srep05685
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000338936400004
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Cited Times [WOS]:19   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Corresponding AuthorPai-Chi Li
Affiliation1.Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei, Taiwan
2.Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan
3.Department of Chemistry and Biochemistry, National Chung-Cheng University, Chia-Yi, Taiwan,
4.Graduate Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan
5.Department of Electrical Engineering, National Taiwan University, Taipei, Taiwan
Recommended Citation
GB/T 7714
Yu-Hsin Wang,Shi-Ping Chen,Ai-Ho Liao,et al. Synergistic delivery of gold nanorods using multifunctional microbubbles for enhanced plasmonic photothermal therapy[J]. Scientific Reports,2014,4:5685.
APA Yu-Hsin Wang.,Shi-Ping Chen.,Ai-Ho Liao.,Ya-Chuen Yang.,Cheng-Ru Lee.,...&Pai-Chi Li.(2014).Synergistic delivery of gold nanorods using multifunctional microbubbles for enhanced plasmonic photothermal therapy.Scientific Reports,4,5685.
MLA Yu-Hsin Wang,et al."Synergistic delivery of gold nanorods using multifunctional microbubbles for enhanced plasmonic photothermal therapy".Scientific Reports 4(2014):5685.
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