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Use of a multiplex enzyme-linked immunosorbent assay to detect a subpopulation of Mycobacterium bovis–infected animals deemed negative or inconclusive by the single intradermal comparative tuberculin skin test
Clare Whelan1; Eduard Shuralev1; Hang Fai Kwok2; Kevin Kenny3; Anthony Duignan3; Margaret Good4; William C. Davis4; John Clarke1
2011
Source PublicationJOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
ISSN1040-6387
Volume23Issue:3Pages:499-503
Abstract

Although the single intradermal comparative tuberculin skin test (SICTT) remains the most effective assay for detecting cattle infected with Mycobacterium bovis, not all infected animals are detected with the SICTT. This has made it difficult to control bovine tuberculosis using a single assay. Use of thegamma interferon assay in conjunction with the SICTT has improved the level of detection but some infected animals still go undetected. This could be in part attributable to both assays being reliant on a cell-mediated immune response. The present study was undertaken to determine if a multiplexassay can improve the level of detection of infected animals when used in combination with theSICTT. The Enferplex TB assay is a multi-antigen ELISA designed for the detection of antibody in animals at different stages of infection and disease. Sixty cattle that were confirmed byhistopathology and/or culture to be infected with M. bovis and that were SICTT negative (43.3%) or difficult to evaluate (56.7% inconclusive) were used in the study. Fifty-three (88.3%) of the animalswere positive in multiplex ELISA. The results show that the level of detection of M. bovis-infectedanimals can be improved by the combined use of the SICTT and the multiplex ELISA.

KeywordMultiplex Bovine Tuberculosis Chemiluminescent Mycobacterium Bovis Single Intradermal Comparative Tuberculin Test
DOIhttp://dx.doi.org/10.1177/1040638711403410
Indexed BySCI
Language英语
WOS Research AreaVeterinary Sciences
WOS SubjectVeterinary Sciences
WOS IDWOS:000294281800010
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Cited Times [WOS]:13   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Enfer Sci, Unit T, Naas, Kildare, Ireland
2.Cent Vet Res Lab, Backweston, Co Dublin, Ireland
3.Dept Agr & Food, Dublin, Ireland
4.Washington State Univ, Coll Vet Med, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA
Recommended Citation
GB/T 7714
Clare Whelan,Eduard Shuralev,Hang Fai Kwok,et al. Use of a multiplex enzyme-linked immunosorbent assay to detect a subpopulation of Mycobacterium bovis–infected animals deemed negative or inconclusive by the single intradermal comparative tuberculin skin test[J]. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION,2011,23(3):499-503.
APA Clare Whelan.,Eduard Shuralev.,Hang Fai Kwok.,Kevin Kenny.,Anthony Duignan.,...&John Clarke.(2011).Use of a multiplex enzyme-linked immunosorbent assay to detect a subpopulation of Mycobacterium bovis–infected animals deemed negative or inconclusive by the single intradermal comparative tuberculin skin test.JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION,23(3),499-503.
MLA Clare Whelan,et al."Use of a multiplex enzyme-linked immunosorbent assay to detect a subpopulation of Mycobacterium bovis–infected animals deemed negative or inconclusive by the single intradermal comparative tuberculin skin test".JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION 23.3(2011):499-503.
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