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Transcriptional Regulation of the Xbr-1a/Xvent-2 Gene by BMP-4 Signaling during Xenopus Embryonic Development
Kim JB; Lee HS; Roh DH; Hwang YS; Xu RH; Kung HF; Bae YC; Park MJ
2000-10
Source PublicationThe Korean Journal of Anatomy
ISSN1225-1305
Volume33Issue:5Pages:595-608
Abstract

BMP-4 signaling is mediated through Smad proteins which may translocate to the nucleus to activate transcription. Little is known about how BMP-4 signaling regulates the transcription of its target genes, e.g., Xvent genes. Therefore, we isolated the genomic clone of a BMP-4 responsive homeobox gene, Xbr-1a/Xvent-2. This clone contains a promoter and three exons for the entire coding region. Using the primer extension, we identified the transcription initiation site corresponding to position -64 bp upstream to the ATG codon of the Xvent-2 gene. The promoter was linked to the luciferase reporter gene, and promoter activity determined by luciferase assay. The temporal promoter activity peaked between embryonic stages 13~17, in agreement with its temporal mRNA expression in the whole embryo. Through the serial deletion mutation, the upstream -235 bp of the promoter retains the full transcriptional activity, and is regulated by BMP-4 signaling. The present results suggest that the BMP-4 responsive element is located on the upstream 235 bp of the promoter.

KeywordEmbryogenesis Gene Transcriptional Regulation Xbr-1a/xvent-2 Xenopus Laevis
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Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Department of Biochemistry, College of Medicine, Hallym University, Chun-Cheon, 200-702, Korea.
2.Department of Anatomy, School of Medicine, KyungPook National University, Taegu, 700-422, Korea.
3.National Institute of Diabetes and Digestive and Kidney Diseases, Laboratory of Biochemistry and Genetics, Bethesda, Maryland 20892, USA
4.Intramural Research Support Program, Science Applications International Corporation-Frederick, Frederick, Maryland 21702-1201, USA.
5.Institute of Molecular Biology, University of Hong Kong, Hong Kong.
Recommended Citation
GB/T 7714
Kim JB,Lee HS,Roh DH,et al. Transcriptional Regulation of the Xbr-1a/Xvent-2 Gene by BMP-4 Signaling during Xenopus Embryonic Development[J]. The Korean Journal of Anatomy,2000,33(5):595-608.
APA Kim JB.,Lee HS.,Roh DH.,Hwang YS.,Xu RH.,...&Park MJ.(2000).Transcriptional Regulation of the Xbr-1a/Xvent-2 Gene by BMP-4 Signaling during Xenopus Embryonic Development.The Korean Journal of Anatomy,33(5),595-608.
MLA Kim JB,et al."Transcriptional Regulation of the Xbr-1a/Xvent-2 Gene by BMP-4 Signaling during Xenopus Embryonic Development".The Korean Journal of Anatomy 33.5(2000):595-608.
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