UM  > 健康科學學院
A microarray platform comparison for neuroscience applications
Parrish M.L.1; Wei N.2; Duenwald S.1; Tokiwa G.Y.1; Wang Y.1; Holder D.2; Dai H.1; Zhang X.2; Wright C.1; Hodor P.2; Cavet G.1; Phillips R.L.2; Sun B.I.2; Fare T.L.1
2004-01-15
Source PublicationJournal of Neuroscience Methods
ISSN01650270
Volume132Issue:1Pages:57-68
AbstractTo address the need for high sensitivity in gene expression profiling of small neural tissue samples (~100ng total RNA), we compared a novel RT-PCR-IVT protocol using fluor-reverse pairs on inkjet oligonucleotide microarrays and an RT-IVT protocol using P labeling on nylon cDNA arrays. The comparison protocol was designed to evaluate these systems for sensitivity, specificity, reproducibility, and linearity. We developed parameters, thresholds, and testing conditions that could be used to differentiate various systems that spanned detection chemistry and instrumentation; probe number and selection criteria; and sample processing protocols. We concluded that the inkjet system had better performance in sensitivity, specificity, and reproducibility than the nylon system, and similar performance in linearity. Between these two platforms, the data indicates that the inkjet system would perform better for the transcriptional profiling of 100ng total RNA samples for neuroscience studies. © 2003 Elsevier B.V. All rights reserved.
KeywordExpression profiling Microarray Nylon membrane Platform comparison Receiver operator characteristic
DOI10.1016/j.jneumeth.2003.09.013
URLView the original
Language英語
Fulltext Access
Citation statistics
Cited Times [WOS]:15   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Rosetta Inpharmatics LLC
2.Merck Research Laboratories
Recommended Citation
GB/T 7714
Parrish M.L.,Wei N.,Duenwald S.,et al. A microarray platform comparison for neuroscience applications[J]. Journal of Neuroscience Methods,2004,132(1):57-68.
APA Parrish M.L..,Wei N..,Duenwald S..,Tokiwa G.Y..,Wang Y..,...&Fare T.L..(2004).A microarray platform comparison for neuroscience applications.Journal of Neuroscience Methods,132(1),57-68.
MLA Parrish M.L.,et al."A microarray platform comparison for neuroscience applications".Journal of Neuroscience Methods 132.1(2004):57-68.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Parrish M.L.]'s Articles
[Wei N.]'s Articles
[Duenwald S.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Parrish M.L.]'s Articles
[Wei N.]'s Articles
[Duenwald S.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Parrish M.L.]'s Articles
[Wei N.]'s Articles
[Duenwald S.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.