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Integrating experimental and analytic approaches to improve data quality in genome-wide RNAi screens
Zhang X.D.; Espeseth A.S.; Johnson E.N.; Chin J.; Gates A.; Mitnaul L.J.; Marine S.D.; Tian J.; Stec E.M.; Kunapuli P.; Holder D.J.; Heyse J.F.; Strulovici B.; Ferrer M.
2008-08-01
Source PublicationJournal of Biomolecular Screening
ISSN10870571 1552454X
Volume13Issue:5Pages:378-389
AbstractRNA interference (RNAi) not only plays an important role in drug discovery but can also be developed directly into drugs. RNAi high-throughput screening (HTS) biotechnology allows us to conduct genome-wide RNAi research. A central challenge in genome-wide RNAi research is to integrate both experimental and computational approaches to obtain high quality RNAi HTS assays. Based on our daily practice in RNAi HTS experiments, we propose the implementation of 3 experimental and analytic processes to improve the quality of data from RNAi HTS biotechnology: (1) select effective biological controls; (2) adopt appropriate plate designs to display and/or adjust for systematic errors of measurement; and (3) use effective analytic metrics to assess data quality. The applications in 5 real RNAi HTS experiments demonstrate the effectiveness of integrating these processes to improve data quality. Due to the effectiveness in improving data quality in RNAi HTS experiments, the methods and guidelines contained in the 3 experimental and analytic processes are likely to have broad utility in genome-wide RNAi research. © 2008 Society for Biomolecular Sciences.
KeywordPlate design Quality control RNAi high-throughput screening Strictly standardized mean difference Z factor
DOI10.1177/1087057108317145
URLView the original
Language英語
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Cited Times [WOS]:26   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
AffiliationMerck Research Laboratories
Recommended Citation
GB/T 7714
Zhang X.D.,Espeseth A.S.,Johnson E.N.,et al. Integrating experimental and analytic approaches to improve data quality in genome-wide RNAi screens[J]. Journal of Biomolecular Screening,2008,13(5):378-389.
APA Zhang X.D..,Espeseth A.S..,Johnson E.N..,Chin J..,Gates A..,...&Ferrer M..(2008).Integrating experimental and analytic approaches to improve data quality in genome-wide RNAi screens.Journal of Biomolecular Screening,13(5),378-389.
MLA Zhang X.D.,et al."Integrating experimental and analytic approaches to improve data quality in genome-wide RNAi screens".Journal of Biomolecular Screening 13.5(2008):378-389.
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