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Genome-Scale RNAi Screen for Host Factors Required for HIV Replication
Zhou H.1; Xu M.1; Huang Q.1; Gates A.T.1; Zhang X.D.1; Castle J.C.2; Stec E.1; Ferrer M.1; Strulovici B.1; Hazuda D.J.1; Espeseth A.S.1
2008-11-13
Source PublicationCell Host and Microbe
ISSN19313128
Volume4Issue:5Pages:495-504
AbstractHuman immunodeficiency virus (HIV)-1 depends on the host cell machinery to support its replication. To discover cellular factors associated with HIV-1 replication, we conducted a genome-scale siRNA screen, revealing more than 311 host factors, including 267 that were not previously linked to HIV. Surprisingly, there was little overlap between these genes and the HIV dependency factors described recently. However, an analysis of the genes identified in both screens revealed overlaps in several of the associated pathways or protein complexes, including the SP1/mediator complex and the NF-κB signaling pathway. cDNAs for a subset of the identified genes were used to rescue HIV replication following knockdown of the cellular mRNA providing strong evidence that the following six genes are previously uncharacterized host factors for HIV: AKT1, PRKAA1, CD97, NEIL3, BMP2K, and SERPINB6. This study highlights both the power and shortcomings of large scale loss-of-function screens in discovering host-pathogen interactions. © 2008 Elsevier Inc. All rights reserved.
KeywordCELLBIO MICROBIO SYSBIO
DOI10.1016/j.chom.2008.10.004
URLView the original
Language英語
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Cited Times [WOS]:516   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
Affiliation1.Merck & Co., Inc.
2.Rosetta Inpharmatics LLC
Recommended Citation
GB/T 7714
Zhou H.,Xu M.,Huang Q.,et al. Genome-Scale RNAi Screen for Host Factors Required for HIV Replication[J]. Cell Host and Microbe,2008,4(5):495-504.
APA Zhou H..,Xu M..,Huang Q..,Gates A.T..,Zhang X.D..,...&Espeseth A.S..(2008).Genome-Scale RNAi Screen for Host Factors Required for HIV Replication.Cell Host and Microbe,4(5),495-504.
MLA Zhou H.,et al."Genome-Scale RNAi Screen for Host Factors Required for HIV Replication".Cell Host and Microbe 4.5(2008):495-504.
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