UM  > 健康科學學院
Genome-wide screens for effective siRNAs through assessing the size of siRNA effects
Zhang X.D.
2008-12-01
Source PublicationBMC Research Notes
ISSN17560500
Volume1
AbstractBackground. RNA interference (RNAi) has been seen as a revolution in functional genomics and system biology. Genome-wide RNAi research relies on the development of RNAi high-throughput screening (HTS) assays. One of the most fundamental challenges in RNAi HTS is to glean biological significance from mounds of data, which relies on the development of effective analytic methods for selecting effective small interfering RNAs (siRNAs). Findings. Based on a recently proposed parameter, strictly standardized mean difference (SSMD), I propose an analytic method for genome-wide screens of effective siRNAs through assessing and testing the size of siRNA effects. Central to this method is the capability of SSMD in quantifying siRNA effects. This method has relied on normal approximation, which works only in the primary screens but not in the confirmatory screens. In this paper, I explore the non-central t-distribution property of SSMD estimates and use this property to extend the SSMD-based method so that it works effectively in either primary or confirmatory screens as well as in any HTS screens with or without replicates. The SSMD-based method maintains a balanced control of false positives and false negatives. Conclusion. The central interest in genome-wide RNAi research is the selection of effective siRNAs which relies on the development of analytic methods to measure the size of siRNA effects. The new analytic method for hit selection provided in this paper offers a good analytic tool for selecting effective siRNAs, better than current analytic methods, and thus may have broad utility in genome-wide RNAi research. © 2008 Zhang; licensee BioMed Central Ltd.
DOI10.1186/1756-0500-1-33
URLView the original
Language英語
全文获取链接
引用统计
Document TypeJournal article
专题Faculty of Health Sciences
AffiliationMerck Research Laboratories
推荐引用方式
GB/T 7714
Zhang X.D.. Genome-wide screens for effective siRNAs through assessing the size of siRNA effects[J]. BMC Research Notes,2008,1.
APA Zhang X.D..(2008).Genome-wide screens for effective siRNAs through assessing the size of siRNA effects.BMC Research Notes,1.
MLA Zhang X.D.."Genome-wide screens for effective siRNAs through assessing the size of siRNA effects".BMC Research Notes 1(2008).
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
Google Scholar
中相似的文章 Google Scholar
[Zhang X.D.]的文章
Baidu academic
中相似的文章 Baidu academic
[Zhang X.D.]的文章
Bing Scholar
中相似的文章 Bing Scholar
[Zhang X.D.]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。