UM  > Faculty of Health Sciences
An effective method for controlling false discovery and false nondiscovery rates in genome-scale RNAi screens
Zhang X.D.
2010-10-01
Source PublicationJournal of Biomolecular Screening
ISSN10870571 1552454X
Volume15Issue:9Pages:1116-1122
AbstractIn most genome-scale RNA interference (RNAi) screens, the ultimate goal is to select siRNAs with a large inhibition or activation effect. The selection of hits typically requires statistical control of 2 errors: false positives and false negatives. Traditional methods of controlling false positives and false negatives do not take into account the important feature in RNAi screens: many small-interfering RNAs (siRNAs) may have very small but real nonzero average effects on the measured response and thus cannot allow us to effectively control false positives and false negatives. To address for deficiencies in the application of traditional approaches in RNAi screening, the author proposes a new method for controlling false positives and false negatives in RNAi high-throughput screens. The false negatives are statistically controlled through a false-negative rate (FNR) or false nondiscovery rate (FNDR). FNR is the proportion of false negatives among all siRNAs examined, whereas FNDR is the proportion of false negatives among declared nonhits. The author also proposes new concepts, q-value and p-value, to control FNR and FNDR, respectively. The proposed method should have broad utility for hit selection in which one needs to control both false discovery and false nondiscovery rates in genome-scale RNAi screens in a robust manner. © 2010 Society for Laboratory Automation and Screening.
Keyword-value false discovery rate false nondiscovery rate p q RNAi high-throughput screening
DOI10.1177/1087057110381783
URLView the original
Language英語
Fulltext Access
Citation statistics
Cited Times [WOS]:15   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionFaculty of Health Sciences
AffiliationMerck Research Laboratories
Recommended Citation
GB/T 7714
Zhang X.D.. An effective method for controlling false discovery and false nondiscovery rates in genome-scale RNAi screens[J]. Journal of Biomolecular Screening,2010,15(9):1116-1122.
APA Zhang X.D..(2010).An effective method for controlling false discovery and false nondiscovery rates in genome-scale RNAi screens.Journal of Biomolecular Screening,15(9),1116-1122.
MLA Zhang X.D.."An effective method for controlling false discovery and false nondiscovery rates in genome-scale RNAi screens".Journal of Biomolecular Screening 15.9(2010):1116-1122.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang X.D.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang X.D.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang X.D.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.