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Screening and expressing HIV-1 specific antibody fragments in Saccharomyces cerevisiae
Wang Y.1; Shan Y.3; Gao X.2,4; Gong R.4; Zheng J.1; Zhang X.D.1; Zhao Q.1
Source PublicationMolecular Immunology
ISSN18729142 01615890

Yeast displaying techniques have been widely used for identifying novel single-chain variable fragments (scFvs) and engineering their binding properties. In this study, we establish a set of vectors for scFv screening and production in the yeast system of Saccharomyces cerevisiae. This suite includes a display vector pYS for screening of recombinant scFv libraries as well as an expression vector pYE for production of scFv candidates in Saccharomyces cerevisiae. The display vector, pYS, give the identification of the HIV-1-specific scFv clones from one scFv display library by fluorescence-activated cell sorting. Subsequently, the expression vector pYE can offer high quality scFvs of interest up to hundreds of microgram scale for bioactivity analysis. As the result, one identified scFv was confirmed to exhibit HIV-1 neutralization activity in a cell line-based pseudovirus assay. The advantage of this system enables the identical post-translation of mammalian scFvs in the same host cells. Therefore, this vector set can be useful for the rapid screening and expression of antibody genes.

KeywordAntibody Expression Hiv-1 Saccharomyces Cerevisiae Yeast Display
URLView the original
Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology ; Immunology
WOS SubjectBiochemistry & Molecular Biology ; Immunology
WOS IDWOS:000450382400031
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Faculty of Health Sciences
Affiliation1.Universidade de Macau
2.University of Chinese Academy of Sciences
3.Jilin University
4.Wuhan Institute of Virology Chinese Academy of Sciences
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GB/T 7714
Wang Y.,Shan Y.,Gao X.,et al. Screening and expressing HIV-1 specific antibody fragments in Saccharomyces cerevisiae[J]. Molecular Immunology,2018,103:279-285.
APA Wang Y..,Shan Y..,Gao X..,Gong R..,Zheng J..,...&Zhao Q..(2018).Screening and expressing HIV-1 specific antibody fragments in Saccharomyces cerevisiae.Molecular Immunology,103,279-285.
MLA Wang Y.,et al."Screening and expressing HIV-1 specific antibody fragments in Saccharomyces cerevisiae".Molecular Immunology 103(2018):279-285.
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