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Simultaneous determination of saponins and fatty acids in Ziziphus jujuba (Suanzaoren) by high performance liquid chromatography-evaporative light scattering detection and pressurized liquid extraction
Zhao J.1; Li S.P.1; Yang F.Q.1; Li P.2; Wang Y.T.1
2006-03-10
Source PublicationJournal of Chromatography A
ISSN00219673
Volume1108Issue:2Pages:188-194
AbstractThe seed of Ziziphus jujube Mill. var. spinosa (Bunge) Hu ex H. F. Chou, Suanzaoren in Chinese, is one of commonly used Chinese medicines. Saponins and fatty oil contains several fatty acids in Suanzaoren are responsible for its therapeutic activities. In this study, a new HPLC coupled with evaporative light scattering detection (ELSD) and pressurized liquid extraction (PLE) method was developed for the simultaneous quantitative determination of 11 major components of 2 saponins and 9 fatty acids, namely jujuboside A, jujuboside B, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid, arachidic acid and docosanoic acid in Suanzaoren. Simultaneous separation of these eleven compounds was achieved on a C18 analytical column. The mobile phase consisted of (A) 0.1% aqueous acetic acid and (B) methanol with 0.1% acetic acid using a gradient elution. The drift tube temperature of ELSD was set at 75°C, and nitrogen flow-rate was 1.8 l/min. All calibration curves showed good linearity (r > 0.9955) within test ranges. This method showed good reproducibility for the quantification of these eleven components in Suanzaoren with intra- and inter-day variations of less than 3.41 and 4.37%, respectively. The validated method was successfully applied to quantify 11 investigated components in nine commercial samples of Suanzaoren. © 2006 Elsevier B.V. All rights reserved.
KeywordFatty acid HPLC-ELSD Pressurized liquid extraction (PLE) Semen Ziziphi Spinosae (Suanzaoren) Spaonin Ziziphus jujube Mill. var. spinosa
DOI10.1016/j.chroma.2005.12.104
URLView the original
Language英語
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被引频次[WOS]:110   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题Institute of Chinese Medical Sciences
Affiliation1.Universidade de Macau
2.China Pharmaceutical University
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Zhao J.,Li S.P.,Yang F.Q.,et al. Simultaneous determination of saponins and fatty acids in Ziziphus jujuba (Suanzaoren) by high performance liquid chromatography-evaporative light scattering detection and pressurized liquid extraction[J]. Journal of Chromatography A,2006,1108(2):188-194.
APA Zhao J.,Li S.P.,Yang F.Q.,Li P.,&Wang Y.T..(2006).Simultaneous determination of saponins and fatty acids in Ziziphus jujuba (Suanzaoren) by high performance liquid chromatography-evaporative light scattering detection and pressurized liquid extraction.Journal of Chromatography A,1108(2),188-194.
MLA Zhao J.,et al."Simultaneous determination of saponins and fatty acids in Ziziphus jujuba (Suanzaoren) by high performance liquid chromatography-evaporative light scattering detection and pressurized liquid extraction".Journal of Chromatography A 1108.2(2006):188-194.
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