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Qualitative and quantitative analysis of specific polysaccharides in Dendrobium huoshanense by using saccharide mapping and chromatographic methods
Deng Y.3; Chen L.-X.3; Han B.-X.1; Wu D.-T.3; Cheong K.-L.3; Chen N.-F.1; Zhao J.3; Li S.-P.3
2016-09-10
Source PublicationJournal of Pharmaceutical and Biomedical Analysis
ISSN1873264X 07317085
Volume129Pages:163-171
AbstractQualitative and quantitative analysis of specific polysaccharides from ten batches of Dendrobium huoshanense were performed using high performance size exclusion chromatography coupled with multi-angle laser light scattering and refractive index detector (HPSEC-MALLS-RID), gas chromatography-mass spectrometry (GC–MS), nuclear magnetic resonance (NMR) and saccharide mapping based on polysaccharides analysis by using carbohydrate gel electrophoresis (PACE) and high performance thin layer chromatography (HPTLC). Results showed that molecular weights, the radius of gyrations, and contents of specific polysaccharides in D. huoshanense were ranging from 1.16 × 10 to 2.17 × 10 Da, 38.8 to 52.1 nm, and 9.9% to 19.9%, respectively. Furthermore, the main monosaccharide compositions were Man and Glc. Indeed, the main glycosidic linkages were β-1,4-Manp and β-1,4-Glcp, and substituted with acetyl groups at O-2 and O-3 of 1,4-linked Manp. Moreover, results showed that PACE and HPTLC fingerprints of partial acidic and enzymatic hydrolysates of specific polysaccharides were similar, which are helpful to better understand the specific polysaccharides in D. huoshanense and beneficial to improve their quality control. These approaches could also be routinely used for quality control of polysaccharides in other medicinal plants.
KeywordDendrobium huoshanense HPSEC-MALLS-RID PACE Saccharide mapping Specific polysaccharides
DOI10.1016/j.jpba.2016.06.051
URLView the original
Language英語
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Cited Times [WOS]:12   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Anhui Collaborative Innovation Center of Dendrobium Industrialization
2.West AnHui University
3.Universidade de Macau
Recommended Citation
GB/T 7714
Deng Y.,Chen L.-X.,Han B.-X.,et al. Qualitative and quantitative analysis of specific polysaccharides in Dendrobium huoshanense by using saccharide mapping and chromatographic methods[J]. Journal of Pharmaceutical and Biomedical Analysis,2016,129:163-171.
APA Deng Y..,Chen L.-X..,Han B.-X..,Wu D.-T..,Cheong K.-L..,...&Li S.-P..(2016).Qualitative and quantitative analysis of specific polysaccharides in Dendrobium huoshanense by using saccharide mapping and chromatographic methods.Journal of Pharmaceutical and Biomedical Analysis,129,163-171.
MLA Deng Y.,et al."Qualitative and quantitative analysis of specific polysaccharides in Dendrobium huoshanense by using saccharide mapping and chromatographic methods".Journal of Pharmaceutical and Biomedical Analysis 129(2016):163-171.
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