Preparation of xylooligosaccharides from xylan by controlled acid hydrolysis and fast protein liquid chromatography coupled with refractive index detection

doi:10.1016/j.seppur.2016.06.051

	Preparation of xylooligosaccharides from xylan by controlled acid hydrolysis and fast protein liquid chromatography coupled with refractive index detection
	Yang Z.-Y.2; Wu D.-T.2; Chen C.-W.1; Cheong K.-L.2; Deng Y.2; Chen L.-X.2; Han B.-X.1; Chen N.-F.1; Zhao J.2; Li S.-P.2
	2016-10-17
Source Publication	Separation and Purification Technology
ISSN	18733794 13835866
Volume	171 Pages:151-156
Abstract	Xylooligosaccharides (XOSs) show many remarkable biological activities and have been widely used in pharmaceuticals, agricultural and functional food-related areas. However, the preparation of pure XOSs standards has not been satisfactory, despite that such standards were crucial for the quality control of XOSs and their products. It is necessary to develop an efficient method for preparation of XOSs. In this study, crude XOSs were obtained by controlled acid hydrolysis of xylan. Parameters of acid hydrolysis such as sulfuric acid concentration, hydrolysis time, and temperature were investigated, allowing optimum conditions to be determined (0.1 M HSO, 90 °C, 120 min). Then the hydrolysates were isolated and separated by FPLC-RID based on anion exchange chromatography (AEC) using DEAE cellulose and size exclusion chromatography (SEC) using Bio-Gel P-2, respectively. Five purified fractions were obtained, and their purities were above 95% determined by using HPTLC and HPLC-CAD. The fractions were also identified as xylooligosaccharides with degrees of polymerization of 2–6 by using MALDI-TOF-MS and GC–MS analysis. The results indicated that the developed FPLC-RID was an efficient and automatic approach for the separation and purification of XOSs with high purity from controlled acid hydrolysis of xylan, which is helpful to quality control of XOSs and their products.
Keyword	Anion exchange chromatography Controlled acid hydrolysis Fast protein liquid chromatography-refractive index detection Size exclusion chromatography Xylooligosaccharides
DOI	10.1016/j.seppur.2016.06.051
URL	View the original
Language	英語
Fulltext Access	View Full-Text via DOI View Full-Text via Web of Science View Full-Text via Scopus
Citation statistics	Cited Times [WOS]:4 [WOS Record] [Related Records in WOS]
Document Type	Journal article
Collection	University of Macau
Affiliation	1.West AnHui University 2.Universidade de Macau
Recommended Citation GB/T 7714	Yang Z.-Y.,Wu D.-T.,Chen C.-W.,et al. Preparation of xylooligosaccharides from xylan by controlled acid hydrolysis and fast protein liquid chromatography coupled with refractive index detection[J]. Separation and Purification Technology,2016,171:151-156.
APA	Yang Z.-Y..,Wu D.-T..,Chen C.-W..,Cheong K.-L..,Deng Y..,...&Li S.-P..(2016).Preparation of xylooligosaccharides from xylan by controlled acid hydrolysis and fast protein liquid chromatography coupled with refractive index detection.Separation and Purification Technology,171,151-156.
MLA	Yang Z.-Y.,et al."Preparation of xylooligosaccharides from xylan by controlled acid hydrolysis and fast protein liquid chromatography coupled with refractive index detection".Separation and Purification Technology 171(2016):151-156.

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