UM
Optimization of microwave-assisted extraction of bioactive alkaloids from lotus plumule using response surface methodology
Xiong W.; Chen X.; Lv G.; Hu D.; Zhao J.; Li S.
2016-12-01
Source PublicationJournal of Pharmaceutical Analysis
ISSN20951779
Volume6Issue:6Pages:382-388
AbstractIn this work, a fast and efficient microwave-assisted extraction (MAE) method was developed to extract main bioactive alkaloids from lotus plumue. To optimize MAE conditions, three main factors were selected using univariate approach experiments, and then central composite design (CCD). The optimal extraction conditions were as follows: methanol concentration of 65%, microwave power of 200 W, and extraction time of 260 s. An high performance liquid chromatography-diode array detector (HPLC-DAD) method was established to quantitatively analyze these phytochemicals in different lotus plumule samples and in different part of lotus. Chromatographic separation was carried out on an Agilent Zorbax Extend-C column (4.6 mm×150 mm, 3.5 µm). Gradient elution was applied with the mobile phase constituted with 0.1% triethylamine in water (A) and acetonitrile (B): 40%−70% B at 0−8 min, 70%−100% B at 8–9 min, 100% B for 2 min, and then equilibrated with 40% B for 2 min.
KeywordAlkaloids Functional food Lotus plumule Microwave-assisted extraction
DOI10.1016/j.jpha.2016.05.007
URLView the original
Language英語
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Cited Times [WOS]:13   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
AffiliationUniversidade de Macau
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GB/T 7714
Xiong W.,Chen X.,Lv G.,et al. Optimization of microwave-assisted extraction of bioactive alkaloids from lotus plumule using response surface methodology[J]. Journal of Pharmaceutical Analysis,2016,6(6):382-388.
APA Xiong W.,Chen X.,Lv G.,Hu D.,Zhao J.,&Li S..(2016).Optimization of microwave-assisted extraction of bioactive alkaloids from lotus plumule using response surface methodology.Journal of Pharmaceutical Analysis,6(6),382-388.
MLA Xiong W.,et al."Optimization of microwave-assisted extraction of bioactive alkaloids from lotus plumule using response surface methodology".Journal of Pharmaceutical Analysis 6.6(2016):382-388.
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