Optimization of microwave-assisted extraction of bioactive alkaloids from lotus plumule using response surface methodology

doi:10.1016/j.jpha.2016.05.007

UM > Institute of Chinese Medical Sciences

	Optimization of microwave-assisted extraction of bioactive alkaloids from lotus plumule using response surface methodology
	Xiong W.; Chen X.; Lv G.; Hu D.; Zhao J.; Li S.
	2016-12-01
Source Publication	Journal of Pharmaceutical Analysis
ISSN	20951779
Volume	6 Issue:6 Pages:382-388
Abstract	In this work, a fast and efficient microwave-assisted extraction (MAE) method was developed to extract main bioactive alkaloids from lotus plumue. To optimize MAE conditions, three main factors were selected using univariate approach experiments, and then central composite design (CCD). The optimal extraction conditions were as follows: methanol concentration of 65%, microwave power of 200 W, and extraction time of 260 s. An high performance liquid chromatography-diode array detector (HPLC-DAD) method was established to quantitatively analyze these phytochemicals in different lotus plumule samples and in different part of lotus. Chromatographic separation was carried out on an Agilent Zorbax Extend-C column (4.6 mm×150 mm, 3.5 µm). Gradient elution was applied with the mobile phase constituted with 0.1% triethylamine in water (A) and acetonitrile (B): 40%−70% B at 0−8 min, 70%−100% B at 8–9 min, 100% B for 2 min, and then equilibrated with 40% B for 2 min.
Keyword	Alkaloids Functional food Lotus plumule Microwave-assisted extraction
DOI	10.1016/j.jpha.2016.05.007
URL	View the original
Language	英語
Fulltext Access	View Full-Text via DOI View Full-Text via Web of Science View Full-Text via Scopus
Citation statistics	Cited Times [WOS]:17 [WOS Record] [Related Records in WOS]
Document Type	Journal article
Collection	Institute of Chinese Medical Sciences
Affiliation	Universidade de Macau
Recommended Citation GB/T 7714	Xiong W.,Chen X.,Lv G.,et al. Optimization of microwave-assisted extraction of bioactive alkaloids from lotus plumule using response surface methodology[J]. Journal of Pharmaceutical Analysis,2016,6(6):382-388.
APA	Xiong W.,Chen X.,Lv G.,Hu D.,Zhao J.,&Li S..(2016).Optimization of microwave-assisted extraction of bioactive alkaloids from lotus plumule using response surface methodology.Journal of Pharmaceutical Analysis,6(6),382-388.
MLA	Xiong W.,et al."Optimization of microwave-assisted extraction of bioactive alkaloids from lotus plumule using response surface methodology".Journal of Pharmaceutical Analysis 6.6(2016):382-388.

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