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Non-starch polysaccharide from Chinese yam activated RAW 264.7 macrophages through the Toll-like receptor 4 (TLR4)-NF-κB signaling pathway
Li M.; Chen L.-X.; Chen S.-R.; Deng Y.; Zhao J.; Wang Y.; Li S.-P.
2017-10-01
Source PublicationJournal of Functional Foods
ISSN17564646
Volume37Pages:491-500
Abstract

A non-starch polysaccharide (NSCYP) isolated and purified from Chinese yam (Dioscorea opposita Thunb.) with its immunomodulatory activities on RAW 264.7 macrophages in vitro was reported. Chemical studies showed that the average molecular weight was 71.78 kDa. NSCYP was mainly composed of mannose, galacturonic acid, glucose, galactose, and arabinose with a molar ratio of 1.09:0.51:1.0:3.03:1.77. Moreover, t-Araf, 1,5-Araf, t-Glcp, t-Galp, t-GalAp, t-GalMEp, 1,4-Galp, 1,4-GalAp, 1,4-GalMEp, 1,4-Glcp, 1,6-Galp, 4,6-Galp and 4,6-Manp were detected in NSCYP. NSCYP promoted RAW 264.7 macrophages phagocytic activity, NO release, transcription and production of interleukin (IL)-6 and tumor necrosis factor (TNF)-α. NSCYP induced NF-κB activation and IκBα degradation in RAW 264.7 cells. Notably, inhibition of Toll-like receptor 4 led to the suppression of NSCYP-induced NO production in RAW 264.7 cells. These findings suggested that NSCYP exhibit the potential as functional food or therapeutic stimulant as a natural immune-stimulating polysaccharide.

KeywordChinese Yam Immunogenic Activity Nf-κb Singling Non-starch Polysaccharide Raw 264.7 Macrophages Toll-like Receptor 4
DOI10.1016/j.jff.2017.08.025
URLView the original
Indexed BySCI
Language英语
WOS Research AreaFood Science & Technology
WOS SubjectFood Science & Technology
WOS IDWOS:000412863400049
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Cited Times [WOS]:7   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Corresponding AuthorLi M.; Chen L.-X.; Chen S.-R.; Deng Y.; Wang Y.; Li S.-P.
AffiliationState Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Avenida da Universidade, Taipa, Macao
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Li M.,Chen L.-X.,Chen S.-R.,et al. Non-starch polysaccharide from Chinese yam activated RAW 264.7 macrophages through the Toll-like receptor 4 (TLR4)-NF-κB signaling pathway[J]. Journal of Functional Foods,2017,37:491-500.
APA Li M..,Chen L.-X..,Chen S.-R..,Deng Y..,Zhao J..,...&Li S.-P..(2017).Non-starch polysaccharide from Chinese yam activated RAW 264.7 macrophages through the Toll-like receptor 4 (TLR4)-NF-κB signaling pathway.Journal of Functional Foods,37,491-500.
MLA Li M.,et al."Non-starch polysaccharide from Chinese yam activated RAW 264.7 macrophages through the Toll-like receptor 4 (TLR4)-NF-κB signaling pathway".Journal of Functional Foods 37(2017):491-500.
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