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A rapid method for simultaneous determination of 14 phenolic compounds in Radix Puerariae using microwave-assisted extraction and ultra high performance liquid chromatography coupled with diode array detection and time-of-flight mass spectrometry
Du G.2; Zhao H.Y.2; Zhang Q.W.2; Li G.H.2; Yang F.Q.3; Wang Y.1; Li Y.C.1; Wang Y.T.2
2010-01-29
Source PublicationJournal of Chromatography A
ISSN00219673
Volume1217Issue:5Pages:705-714
AbstractA microwave-assisted extraction (MAE) and ultra high performance liquid chromatography coupled with diode array detection and time-of-flight mass spectrometry (UHPLC-DAD-TOF-MS) method was developed for simultaneous determination of 14 phenolic compounds in the root of Pueraria lobata (Wild.) Ohwi and Pueraria thomsonii Benth. Operational conditions of MAE were optimized by central composite design (CCD). The optimized result was 65% ethanol as extraction solvent, 17 mL of extraction volume, 100 °C of extraction temperature and 2 min of hold time. A Zorbax SB C (50 mm × 4.6 mm I.D., 1.8 μm) and gradient elution were used during the analysis. The chromatographic peaks of 14 investigated compounds in samples were successfully identified by comparing their retention time, UV spectra and TOF mass data with the reference substances. All calibration curves showed good linearity (r > 0.9997) within the test ranges. The intra-day and inter-day variations were less than 1.77% and 2.88%, respectively. The developed method was successfully applied to determine the investigated compounds in 10 samples of Radix Puerariae Lobatae and Radix Puerariae Thomsonii, respectively. The result indicated that MAE and UHPLC-DAD-TOF-MS system might provide a rapid method for the quality control of Radix Puerariae.
KeywordCentral composite design Microwave-assisted extraction Radix Puerariae Time-of-flight mass spectrometry Ultra high performance liquid chromatography
DOI10.1016/j.chroma.2009.12.017
URLView the original
Language英語
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Cited Times [WOS]:78   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Sichuan Provincial Institute for Food and Drug Control
2.Universidade de Macau
3.Chongqing University
Recommended Citation
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Du G.,Zhao H.Y.,Zhang Q.W.,et al. A rapid method for simultaneous determination of 14 phenolic compounds in Radix Puerariae using microwave-assisted extraction and ultra high performance liquid chromatography coupled with diode array detection and time-of-flight mass spectrometry[J]. Journal of Chromatography A,2010,1217(5):705-714.
APA Du G..,Zhao H.Y..,Zhang Q.W..,Li G.H..,Yang F.Q..,...&Wang Y.T..(2010).A rapid method for simultaneous determination of 14 phenolic compounds in Radix Puerariae using microwave-assisted extraction and ultra high performance liquid chromatography coupled with diode array detection and time-of-flight mass spectrometry.Journal of Chromatography A,1217(5),705-714.
MLA Du G.,et al."A rapid method for simultaneous determination of 14 phenolic compounds in Radix Puerariae using microwave-assisted extraction and ultra high performance liquid chromatography coupled with diode array detection and time-of-flight mass spectrometry".Journal of Chromatography A 1217.5(2010):705-714.
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