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Novel Kunitz-like Peptides Discovered in the Zoanthid Palythoa caribaeorum through Transcriptome Sequencing
Liao, Qiwen1; Li, Shengnan1; Siu, Shirley Weng In2; Yang, Binrui1; Huang, Chen1; Chan, Judy Yuet-Wa1; Morlighem, Jean-Etienne R. L.3; Wong, Clarence Tsun Ting4; Radis-Baptista, Gandhi3; Lee, Simon Ming-Yuen1
2018-02
Source PublicationJOURNAL OF PROTEOME RESEARCH
ISSN1535-3893
Volume17Issue:2Pages:891-902
Abstract

Palythoa caribaeorum (class Anthozoa) is a zoanthid that together jellyfishes, hydra, and sea anemones, which are venomous and predatory, belongs to the Phyllum Cnidaria. The distinguished feature in these marine animals is the cnidocytes in the body tissues, responsible for toxin production and injection that are used majorly for prey capture and defense. With exception for other anthozoans, the toxin cocktails of zoanthids have been scarcely studied and are poorly known. Here, on the basis of the analysis of P. caribaeorum transcriptome, numerous predicted venom featured polypeptides were identified including allergens, neurotoxins, membrane-active, and Kunitz-like peptides (PcKuz). The three predicted PcKuz isotoxins (1-3) were selected for functional studies. Through computational processing comprising structural phylogenetic analysis, molecular docking, and dynamics simulation, PcKuz3 was shown to be a potential voltage gated potassium-channel inhibitor. PcKuz3 fitted well as new functional Kunitz-type toxins with strong antilocomotor activity as in vivo assessed in zebrafish larvae, with weak inhibitory effect toward proteases, as evaluated in vitro. Notably, PcKuz3 can suppress, at low concentration, the 6-OHDA-induced neurotoxicity on the locomotive behavior of zebrafish, which indicated PcKuz3 may have a neuroprotective effect. Taken together, PcKuz3 figures as a novel neurotoxin structure, which differs from known homologous peptides expressed in sea anemone. Moreover, the novel PcKuz3 provides an insightful hint for biodrug development for prospective neurodegenerative disease treatment.

KeywordSoft Coral Transcriptome Kunitz-like Peptides Protein Docking Zebrafish Neurotoxin Zoanthids
DOI10.1021/acs.jproteome.7b00686
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000424730700013
PublisherAMER CHEMICAL SOC
The Source to ArticleWOS
Fulltext Access
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Cited Times [WOS]:5   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionDEPARTMENT OF COMPUTER AND INFORMATION SCIENCE
Institute of Chinese Medical Sciences
Corresponding AuthorRadis-Baptista, Gandhi; Lee, Simon Ming-Yuen
Affiliation1.State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Macau, China
2.Department of Computer and Information Science, Faculty of Science and Technology, University of Macau, Macau, China
3.Laboratory of Biochemistry and Biotechnology, Institute for Marine Sciences, Federal University of Ceará, Fortaleza 60020-181, Brazil
4.Department of Chemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China
First Author AffilicationInstitute of Chinese Medical Sciences
Corresponding Author AffilicationInstitute of Chinese Medical Sciences
Recommended Citation
GB/T 7714
Liao, Qiwen,Li, Shengnan,Siu, Shirley Weng In,et al. Novel Kunitz-like Peptides Discovered in the Zoanthid Palythoa caribaeorum through Transcriptome Sequencing[J]. JOURNAL OF PROTEOME RESEARCH,2018,17(2):891-902.
APA Liao, Qiwen.,Li, Shengnan.,Siu, Shirley Weng In.,Yang, Binrui.,Huang, Chen.,...&Lee, Simon Ming-Yuen.(2018).Novel Kunitz-like Peptides Discovered in the Zoanthid Palythoa caribaeorum through Transcriptome Sequencing.JOURNAL OF PROTEOME RESEARCH,17(2),891-902.
MLA Liao, Qiwen,et al."Novel Kunitz-like Peptides Discovered in the Zoanthid Palythoa caribaeorum through Transcriptome Sequencing".JOURNAL OF PROTEOME RESEARCH 17.2(2018):891-902.
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