UM
Simultaneous quantification of coumarins, flavonoids and limonoids in Fructus Citri Sarcodactylis by high performance liquid chromatography coupled with diode array detector
Chu J.1; Li S.-L.1; Yin Z.-Q.4; Ye W.-C.3; Zhang Q.-W.1
2012-07-01
Source PublicationJournal of Pharmaceutical and Biomedical Analysis
ISSN07317085 1873264X
Volume66Pages:170-175
AbstractA high performance liquid chromatography coupled with diode array detector (HPLC-DAD) method was developed for simultaneous quantification of eleven major bioactive components including six coumarins, three flavonoids and two limonoids in Fructus Citri Sarcodactylis. The analysis was performed on a Cosmosil 5 C -MS-II column (4.6mm×250mm, 5μm) with water-acetonitrile gradient elution. The method was validated in terms of linearity, sensitivity, precision, stability and accuracy. It was found that the calibration curves for all analytes showed good linearity (R >0.9993) within the test ranges. The overall limit of detection (LOD) and limit of quantification (LOQ) were less than 3.0 and 10.2ng. The relative standard deviations (RSDs) for intra- and inter-day repeatability were not more than 4.99% and 4.92%, respectively. The sample was stable for at least 48h. The spike recoveries of eleven components were 95.1-104.9%. The established method was successfully applied to determine eleven components in three samples from different locations. The results showed that the newly developed HPLC-DAD method was linear, sensitive, precise and accurate, and could be used for quality control of Fructus Citri Sarcodactylis. © 2012 Elsevier B.V.
KeywordCitrus medica var. sarcodactylis Coumarin Flavonoid HPLC-DAD Limonoid
DOI10.1016/j.jpba.2012.03.041
URLView the original
Language英語
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Cited Times [WOS]:13   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Universidade de Macau
2.Jiangsu Province Academy of Chinese Medicine
3.Jinan University
4.China Pharmaceutical University
Recommended Citation
GB/T 7714
Chu J.,Li S.-L.,Yin Z.-Q.,et al. Simultaneous quantification of coumarins, flavonoids and limonoids in Fructus Citri Sarcodactylis by high performance liquid chromatography coupled with diode array detector[J]. Journal of Pharmaceutical and Biomedical Analysis,2012,66:170-175.
APA Chu J.,Li S.-L.,Yin Z.-Q.,Ye W.-C.,&Zhang Q.-W..(2012).Simultaneous quantification of coumarins, flavonoids and limonoids in Fructus Citri Sarcodactylis by high performance liquid chromatography coupled with diode array detector.Journal of Pharmaceutical and Biomedical Analysis,66,170-175.
MLA Chu J.,et al."Simultaneous quantification of coumarins, flavonoids and limonoids in Fructus Citri Sarcodactylis by high performance liquid chromatography coupled with diode array detector".Journal of Pharmaceutical and Biomedical Analysis 66(2012):170-175.
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