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Multi-ingredients determination and fingerprint analysis of leaves from Ilex latifolia using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry
Fan C.3; Deng J.1; Yang Y.1; Liu J.3; Wang Y.3; Zhang X.3; Fai K.2; Zhang Q.4; Ye W.3
2013-10-01
Source PublicationJournal of Pharmaceutical and Biomedical Analysis
ISSN07317085 1873264X
Volume84Pages:20-29
Abstract

An ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) method integrating multi-ingredients determination and fingerprint analysis has been established for quality assessment and control of leaves from Ilex latifolia. The method possesses the advantages of speediness, efficiency, accuracy, and allows the multi-ingredients determination and fingerprint analysis in one chromatographic run within 13. min. Multi-ingredients determination was performed based on the extracted ion chromatograms of the exact pseudo-molecular ions (with a 0.01. Da window), and fingerprint analysis was performed based on the base peak chromatograms, obtained by negative-ion electrospray ionization QTOF-MS. The method validation results demonstrated our developed method possessing desirable specificity, linearity, precision and accuracy. The method was utilized to analyze 22 I. latifolia samples from different origins. The quality assessment was achieved by using both similarity analysis (SA) and principal component analysis (PCA), and the results from SA were consistent with those from PCA. Our experimental results demonstrate that the strategy integrated multi-ingredients determination and fingerprint analysis using UPLC-QTOF-MS technique is a useful approach for rapid pharmaceutical analysis, with promising prospects for the differentiation of origin, the determination of authenticity, and the overall quality assessment of herbal medicines. © 2013 Elsevier B.V.

KeywordFingerprint Analysis Ilex Latifolia Multi-ingredients Determination Quadrupole Time-of-flight Mass Spectrometry Ultra-performance Liquid Chromatography
DOI10.1016/j.jpba.2013.05.039
URLView the original
Indexed BySCI
WOS Research AreaChemistry ; Pharmacology & Pharmacy
WOS SubjectChemistry, Analytical ; Pharmacology & Pharmacy
WOS IDWOS:000322752800004
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Cited Times [WOS]:19   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.China National Analytical Center Guangzhou
2.Macao Polytechnic Institute
3.Jinan University
4.Universidade de Macau
Recommended Citation
GB/T 7714
Fan C.,Deng J.,Yang Y.,et al. Multi-ingredients determination and fingerprint analysis of leaves from Ilex latifolia using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry[J]. Journal of Pharmaceutical and Biomedical Analysis,2013,84:20-29.
APA Fan C..,Deng J..,Yang Y..,Liu J..,Wang Y..,...&Ye W..(2013).Multi-ingredients determination and fingerprint analysis of leaves from Ilex latifolia using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.Journal of Pharmaceutical and Biomedical Analysis,84,20-29.
MLA Fan C.,et al."Multi-ingredients determination and fingerprint analysis of leaves from Ilex latifolia using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry".Journal of Pharmaceutical and Biomedical Analysis 84(2013):20-29.
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