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Simultaneous determination of eight flavonoids and pogostone in pogostemon cablin by high performance liquid chromatography
Li P.3; Yin Z.-Q.4; Li S.-L.3; Huang X.-J.3; Ye W.-C.2; Zhang Q.-W.3
2014-07-21
Source PublicationJournal of Liquid Chromatography and Related Technologies
ISSN1520572X 10826076
Volume37Issue:12Pages:1771-1784
Abstract

A high performance liquid chromatography (HPLC) method was developed and validated for simultaneous quantification of nine major components including volatile and nonvolatile ingredients in Pogostemon cablin (Blanco) Benth. The analysis was performed on a Cosmosil 5 C18-AR-II column (4.6 mm - 250 mm, 5 μm) with 0.2% formic acid in water-acetonitrile gradient elution. The method was validated in terms of linearity, sensitivity, precision, stability, and accuracy. It was found that the calibration curves for all analytes showed good linearity (R2 > 0.9991) within the test ranges. The overall limit of detection (LOD) and limit of quantification (LOQ) ere less than 36.32 and 122.09 ng. The relative standard deviations (RSDs) for intra-day and inter-day repeatability were no more than 3.23% and 4.85%, respectively. The sample was stable for at least 48 hr. The spike recoveries of nine components were 96-106%. The established method was successfully applied to determine nine components in thirteen samples from different locations. The results showed that the newly developed HPLC-DAD method was sensitive, precise, and accurate and could be used for quality control of P. cablin. © 2014 Copyright Taylor & Francis Group, LLC.

KeywordFlavonoids Hplc-dad Pogostemon Cablin Pogostone Quantification Simultaneous Determination
DOI10.1080/10826076.2013.809545
URLView the original
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000334042700011
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被引频次[WOS]:8   [WOS记录]     [WOS相关记录]
Document TypeJournal article
专题Institute of Chinese Medical Sciences
Affiliation1.Jiangsu Province Academy of Traditional Chinese Medicine
2.Jinan University
3.Universidade de Macau
4.China Pharmaceutical University
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Li P.,Yin Z.-Q.,Li S.-L.,et al. Simultaneous determination of eight flavonoids and pogostone in pogostemon cablin by high performance liquid chromatography[J]. Journal of Liquid Chromatography and Related Technologies,2014,37(12):1771-1784.
APA Li P.,Yin Z.-Q.,Li S.-L.,Huang X.-J.,Ye W.-C.,&Zhang Q.-W..(2014).Simultaneous determination of eight flavonoids and pogostone in pogostemon cablin by high performance liquid chromatography.Journal of Liquid Chromatography and Related Technologies,37(12),1771-1784.
MLA Li P.,et al."Simultaneous determination of eight flavonoids and pogostone in pogostemon cablin by high performance liquid chromatography".Journal of Liquid Chromatography and Related Technologies 37.12(2014):1771-1784.
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