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Cytotoxic and apoptosis-inducing activity of C21 steroids from the roots of Cynanchum atratum
Zhang J.1; Ma L.1; Wu Z.-F.1; Yu S.-L.1; Wang L.2; Ye W.-C.2; Zhang Q.-W.3; Yin Z.-Q.4
2017
Source PublicationSteroids
ISSN18785867 0039128X
Volume122Pages:1-8
AbstractTwo new (1–2) and two known C steroids (3–4) were isolated from the roots of Cynanchum atratum. Their structures were elucidated by detailed 1D and 2D spectroscopic. The MTT assay showed that compounds 1–4 displayed obvious cytotoxic activities against HepG2 cells with IC values ranging from 10.19 μM to 76.12 μM. Compounds 1–3 also exhibited cytotoxic effects in A549 cells with IC values of 30.87–95.39 μM. Compound 3 showed the antiproliferative activity via G0/G1 cell cycle arrest and proapoptosis in HepG2 cells by Flowcytometry analysis. Western blotting analysis revealed that compound 3 could induce HepG2 cell apoptosis via the mitochondrial pathway by downregulating Bcl-2 expression, upregulating Bax protein expression, and activating caspase-9 and caspase-3.
KeywordApoptosis C21 steroids Cynanchum atratum Cytotoxic activity Mitochondrial pathway
DOI10.1016/j.steroids.2017.03.004
URLView the original
Language英語
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Cited Times [WOS]:8   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Jiangsu Province Academy of Traditional Chinese Medicine
2.Jinan University
3.Universidade de Macau
4.China Pharmaceutical University
5.Nanjing University of Traditional Chinese Medicine
Recommended Citation
GB/T 7714
Zhang J.,Ma L.,Wu Z.-F.,et al. Cytotoxic and apoptosis-inducing activity of C21 steroids from the roots of Cynanchum atratum[J]. Steroids,2017,122:1-8.
APA Zhang J..,Ma L..,Wu Z.-F..,Yu S.-L..,Wang L..,...&Yin Z.-Q..(2017).Cytotoxic and apoptosis-inducing activity of C21 steroids from the roots of Cynanchum atratum.Steroids,122,1-8.
MLA Zhang J.,et al."Cytotoxic and apoptosis-inducing activity of C21 steroids from the roots of Cynanchum atratum".Steroids 122(2017):1-8.
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