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1H nuclear magnetic resonance based-metabolomic characterization of Peucedani Radix and simultaneous determination of praeruptorin A and praeruptorin B
Song Y.-L.1; Jing W.-H.1; Chen Y.-G.1; Yuan Y.-F.2; Yan R.1; Wang Y.-T.1
2014
Source PublicationJournal of Pharmaceutical and Biomedical Analysis
ISSN1873264X 07317085
Volume93Pages:86-94
AbstractAs a widely used traditional herbal medicine, it is crucial to characterize the holistic metabolic profile of Peucedani Radix (Chinese name: Qian-hu). However, it is quite arduous to obtain the whole picture of chemical constituents appropriately with the existing analytical techniques that were based on HPLC-UV or LC-MS/MS system. In present investigation, nuclear magnetic resonance (NMR) spectroscopy coupled with principal components analysis (PCA) was introduced to metabolomic characterization of Qian-hu crude extracts without any chromatographic separation. In addition, the contents of praeruptorin A (PA) and proaeruptorin B (PB) in Qian-hu were simultaneously determined using quantitative H NMR (qH NMR) spectroscopy. Eighteen reference compounds (1-18), which were purified from this herbal drug extract previously, were recruited for the assignment of the protonic signals in the H NMR spectra. Following PCA, 15 batches of Peucedani Radix were divided into two groups (I and II), and angular-type pyranocoumarins, in particular PA and PB, as well as 5-methoxycoumarin were demonstrated as the predominant markers being responsible for the distinguishment of Qian-hu from different districts. The contents of the two analytes (PA & PB) were calculated by the relative ratio of the integral values of the target peak for each compound to the known amount of the internal standard, formononetin (IS). The lower limits of quantitation were determined as 19.5μg/mL for both PA and PB. The quantitative results indicated that the contents of PA and PB showed quite variable qualities among different extract samples. Above all, H NMR spectroscopy, that could not only provide comprehensive profiles of the metabolites but also achieve convenient determination of praeruptorin A and praeruptorin B, is a promising means for evaluating the medicinal samples of Peucedani Radix. © 2013.
KeywordMetabolomic profile Peucedani Radix Praeruptorin A Praeruptorin B
DOI10.1016/j.jpba.2013.08.021
URLView the original
Language英語
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Cited Times [WOS]:15   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionInstitute of Chinese Medical Sciences
Affiliation1.Universidade de Macau
2.South China Agricultural University
Recommended Citation
GB/T 7714
Song Y.-L.,Jing W.-H.,Chen Y.-G.,et al. 1H nuclear magnetic resonance based-metabolomic characterization of Peucedani Radix and simultaneous determination of praeruptorin A and praeruptorin B[J]. Journal of Pharmaceutical and Biomedical Analysis,2014,93:86-94.
APA Song Y.-L.,Jing W.-H.,Chen Y.-G.,Yuan Y.-F.,Yan R.,&Wang Y.-T..(2014).1H nuclear magnetic resonance based-metabolomic characterization of Peucedani Radix and simultaneous determination of praeruptorin A and praeruptorin B.Journal of Pharmaceutical and Biomedical Analysis,93,86-94.
MLA Song Y.-L.,et al."1H nuclear magnetic resonance based-metabolomic characterization of Peucedani Radix and simultaneous determination of praeruptorin A and praeruptorin B".Journal of Pharmaceutical and Biomedical Analysis 93(2014):86-94.
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