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Generation of human induced pluripotent stem cells from umbilical cord matrix and amniotic membrane mesenchymal cells
Cai J.2; Li W.2; Su H.5; Qin D.2; Yang J.2; Zhu F.2; Xu J.2; He W.2; Guo X.2; Labuda K.3; Peterbauer A.1; Wolbank S.3; Zhong M.4; Li Z.2; Wu T.5; So K.-F.5; Redl H.3; Zeng L.2; Esteban M.A.2; Pei D.2
2010-04-09
Source PublicationJournal of Biological Chemistry
ISSN00219258 1083351X
Volume285Issue:15Pages:11227-11234
Abstract

The umbilical cord and placenta are extra-embryonic tissues of particular interest for regenerative medicine. They share an early developmental origin and are a source of vast amounts of cells with multilineage differentiation potential that are poorly immunogenic and without controversy. Moreover, these cells are likely exempt from incorporated mutations when compared with juvenile or adult donor cells such as skin fibroblasts or keratinocytes. Here we report the efficient generation of induced pluripotent stem cells (iPSCs) from mesenchymal cells of the umbilical cord matrix (up to 0.4% of the cells became reprogrammed) and the placental amniotic membrane (up to 0.1%) using exogenous factors and a chemical mixture. iPSCs from these 2 tissues homogeneously showed human embryonic stem cell (hESC)-like characteristics including morphology, positive staining for alkaline phosphatase, normal karyotype, and expression of hESC-like markers including Nanog, Rex1, Oct4, TRA-1-60, TRA-1-80, SSEA-3, and SSEA-4. Selected clones also formed embryonic bodies and teratomas containing derivatives of the 3 germ layers, and could as well be readily differentiated into functional motor neurons. Among other things, our cell lines may prove useful for comparisons between iPSCs derived from multiple tissues regarding the extent of the epigenetic reprogramming, differentiation ability, stability of the resulting lineages, and the risk of associated abnormalities.

DOI10.1074/jbc.M109.086389
URLView the original
Indexed BySCIE
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000276286200028
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Cited Times [WOS]:116   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionPersonal research not belonging to the institution
Affiliation1.Austrian Cluster for Tissue Regeneration
2.Chinese Academy of Sciences
3.Ludwig Boltzmann Institute
4.Nanfang Hospital
5.The University of Hong Kong Li Ka Shing Faculty of Medicine
Recommended Citation
GB/T 7714
Cai J.,Li W.,Su H.,et al. Generation of human induced pluripotent stem cells from umbilical cord matrix and amniotic membrane mesenchymal cells[J]. Journal of Biological Chemistry,2010,285(15):11227-11234.
APA Cai J..,Li W..,Su H..,Qin D..,Yang J..,...&Pei D..(2010).Generation of human induced pluripotent stem cells from umbilical cord matrix and amniotic membrane mesenchymal cells.Journal of Biological Chemistry,285(15),11227-11234.
MLA Cai J.,et al."Generation of human induced pluripotent stem cells from umbilical cord matrix and amniotic membrane mesenchymal cells".Journal of Biological Chemistry 285.15(2010):11227-11234.
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