UM
Assessment of the rate of spinal motor axon regeneration by choline acetyltransferase immunohistochemistry following sciatic nerve crush injury in mice: Laboratory investigation
Yuan Q.2; Su H.4; Chiu K.1; Lin Z.-X.2; Wu W.1
2014-02-01
Source PublicationJournal of Neurosurgery
ISSN00223085 19330693
Volume120Issue:2Pages:502-508
AbstractObject. The purpose of this study was to examine whether choline acetyl transferase (ChAT) staining can be used for assessing the rate of motor neuron regeneration at an early phase of axon outgrowth. Methods. The authors developed a new sciatic nerve crush model in adult mice. In this model, in addition to performing a sciatic nerve crush injury, the authors excised the ipsilateral lumbar L3-6 dorsal root ganglion (DRG), which resulted in degeneration of the sensory fibers entering into the sciatic nerve. Crushed nerve sections obtained at Day 3 or Day 7 postinjury were analyzed by means of immunostaining. Results. The immunostaining showed that ChAT, a motor axon-specific antigen, was totally co-localized with growth-associated protein 43 (GAP-43), which is expressed in regenerating nerves and transported into growth cones. Conclusions. Our results suggest that measuring the length of motor axon outgrowth by ChAT immunostaining is reliable. ChAT staining provides a more convenient method for evaluating the rate of motor axon outgrowth in a mixed nerve. ©AANS, 2014.
KeywordAxon regeneration ChAT staining Motor neuron Nerve injury Peripheral nerve
DOI10.3171/2013.8.JNS121648
URLView the original
Language英語
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Cited Times [WOS]:3   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.The University of Hong Kong Li Ka Shing Faculty of Medicine
2.Chinese University of Hong Kong
3.Jinan University
4.Universidade de Macau
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GB/T 7714
Yuan Q.,Su H.,Chiu K.,et al. Assessment of the rate of spinal motor axon regeneration by choline acetyltransferase immunohistochemistry following sciatic nerve crush injury in mice: Laboratory investigation[J]. Journal of Neurosurgery,2014,120(2):502-508.
APA Yuan Q.,Su H.,Chiu K.,Lin Z.-X.,&Wu W..(2014).Assessment of the rate of spinal motor axon regeneration by choline acetyltransferase immunohistochemistry following sciatic nerve crush injury in mice: Laboratory investigation.Journal of Neurosurgery,120(2),502-508.
MLA Yuan Q.,et al."Assessment of the rate of spinal motor axon regeneration by choline acetyltransferase immunohistochemistry following sciatic nerve crush injury in mice: Laboratory investigation".Journal of Neurosurgery 120.2(2014):502-508.
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