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Neural progenitor cell apoptosis and differentiation were affected by activated microglia in spinal cord slice culture
Liu X.1; Chu T.-H.1; Su H.1; Guo A.1; Wu W.1
2014-03-01
Source PublicationNeurological Sciences
ISSN15901874 15903478
Volume35Issue:3Pages:415-419
AbstractNeural progenitor cell (NPC) transplantation offers great potential to treat spinal cord injury (SCI). NPCs may replace lost neurons or oligodendrocytes and act as a source of neurotrophic factors to support survival of remaining cells. However, their efficiency was limited by poor survival after transplantation, and they tended more to differentiate into astrocytes, but not neurons and oligodendrocytes. This study investigated whether activated microglia is a factor that contributes to this phenomenon. Organotypic spinal cord slice (SCS) culture was used to mimic the local environment after SCI, and NPCs were co-cultured with them to share the culture medium. After specific depletion of microglia in the SCSs with clodronate loaded liposome, the apoptotic rate of NPCs decreased, more NPCs differentiated into neurons, and glial differentiation was impaired. This suggested that microglia may impair NPC survival, and neuronal differentiation, but improve astrocyte differentiation. In NPC transplantation strategy for SCI, microglia would be manipulated to improve the survival and neuronal differentiation of NPCs. © 2013 Springer-Verlag Italia.
KeywordMicroglia Neural progenitor cells Neuronal differentiation Spinal cord injury
DOI10.1007/s10072-013-1532-4
URLView the original
Language英語
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Cited Times [WOS]:6   [WOS Record]     [Related Records in WOS]
Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.The University of Hong Kong Li Ka Shing Faculty of Medicine
2.The University of Hong Kong
3.Jinan University
Recommended Citation
GB/T 7714
Liu X.,Chu T.-H.,Su H.,et al. Neural progenitor cell apoptosis and differentiation were affected by activated microglia in spinal cord slice culture[J]. Neurological Sciences,2014,35(3):415-419.
APA Liu X.,Chu T.-H.,Su H.,Guo A.,&Wu W..(2014).Neural progenitor cell apoptosis and differentiation were affected by activated microglia in spinal cord slice culture.Neurological Sciences,35(3),415-419.
MLA Liu X.,et al."Neural progenitor cell apoptosis and differentiation were affected by activated microglia in spinal cord slice culture".Neurological Sciences 35.3(2014):415-419.
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