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Reprogramming somatic cells to cells with neuronal characteristics by defined medium both in vitro and in vivo
He S.1; Guo Y.1; Zhang Y.1; Li Y.1; Feng C.1; Li X.1; Lin L.1; Guo L.1; Wang H.1; Liu C.1; Zheng Y.1; Luo C.2; Liu Q.2; Wang F.1; Sun H.1; Liang L.1; Li L.1; Su H.2; Chen J.1; Pei D.1; Zheng H.1
2015-12-30
Source PublicationCell Regeneration
ISSN20459769
Volume4Issue:1
AbstractBackground: Currently, direct conversion from somatic cells to neurons requires virus-mediated delivery of at least one transcriptional factor or a combination of several small-molecule compounds. Delivery of transcriptional factors may affect genome stability, while small-molecule compounds may require more evaluations when applied in vivo. Thus, a defined medium with only conventional growth factors or additives for cell culture is desirable for inducing neuronal trans-differentiation. Results: Here, we report that a defined medium (5C) consisting of basic fibroblast growth factor (bFGF), N2 supplement, leukemia inhibitory factor, vitamin C (Vc), and β-mercaptoethanol (βMe) induces the direct conversion of somatic cells to cells with neuronal characteristics. Application of 5C medium converted mouse embryonic fibroblasts (MEFs) into TuJ+ neuronal-like cells, which were capable of survival after being transplanted into the mouse brain. The same 5C medium could convert primary rat astrocytes into neuronal-like cells with mature electrophysiology characteristics in vitro and facilitated the recovery of brain injury, possibly by inducing similar conversions, when infused into the mouse brain in vivo. Crucially, 5C medium could also induce neuronal characteristics in several human cell types. Conclusions: In summary, this 5C medium not only provides a means to derive cells with neuronal characteristics without viral transfection in vitro but might also be useful to produce neurons in vivo for neurodegenerative disease treatment.
KeywordAstrocytes Defined medium Neurons Somatic cells Trans-differentiation
DOI10.1186/s13619-015-0027-6
URLView the original
Language英語
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Document TypeJournal article
CollectionUniversity of Macau
Affiliation1.Chinese Academy of Sciences
2.Universidade de Macau
3.Anhui University
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GB/T 7714
He S.,Guo Y.,Zhang Y.,et al. Reprogramming somatic cells to cells with neuronal characteristics by defined medium both in vitro and in vivo[J]. Cell Regeneration,2015,4(1).
APA He S..,Guo Y..,Zhang Y..,Li Y..,Feng C..,...&Zheng H..(2015).Reprogramming somatic cells to cells with neuronal characteristics by defined medium both in vitro and in vivo.Cell Regeneration,4(1).
MLA He S.,et al."Reprogramming somatic cells to cells with neuronal characteristics by defined medium both in vitro and in vivo".Cell Regeneration 4.1(2015).
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